中文摘要：

为了研究小麦谷氨酰胺合成酶（Glutamine synthetase，GS）的功能，利用RACE及RT-PCR技术克隆了小麦GSl（GenBank登录号：HQ840647）和GS2（GenBank登录号：JF894116）基因的全长cDNA．小麦GS1的cDNA全长为1494bp，GS2的cDNA全长为1631bp，并利用生物信息软件对GS1和GS2的基因序列以及所表达的蛋白特性进行了分析．利用半定量PCR和Western-bolt技术分别分析了小麦苗期叶片发育过程中GS同工酶基因转录和表达特点，结果表明，GS1在叶片开始衰老时转录和表达水平较高，GS2从叶片伸长期到定长期转录和表达水平最高．

英文摘要：

In order to study the function of glutamine synthetase （GS） in wheat, the full-length cDNA of GS1 and GS2 were cloned by RACE and RT-PCR （The accession number of GS1 is HQ840647, and GS2 is JF894116）. The full length cDNA of GS1 is 1 494 bp and that of GS2 is 1 631 bp. The se-quences and protein characteristics of GS1 and GS2 were analyzed with the biological software. Semi-quantitative PCR and western-blot were used to demonstrate the transcriptional and expressional levels of GS genes in wheat leaf during seedling stage, and the results showed that both levels of GS1 got high-er when the leaf began to senescence while both levels of GS2 kept the highest from the leaf expanding stage to the full expansion stage.