中文摘要：

目的探讨同源不同辐射抗拒鼻咽癌（nasopharyngeal carcinoma,NPC）细胞DNA甲基化差异,期望从表观遗传学角度阐述人鼻咽癌辐射抗拒的发生机制。方法采用Me DIP-Seq测序法对CNE2R/CNE2细胞进行测序,分析比较DNA甲基化水平差异区间的分布。结果 CNE-2R和CNE-2细胞甲基化分布差异主要集中在第5、6、7、9、13、17号染色体上,其中第5、6、13、17号染色体上为甲基化上调区间的密集区域,第7、9号染色体上为甲基化下调的密集区域。结论同源不同辐射抗拒鼻咽癌细胞CNE-2R和CNE-2细胞的甲基化水平存在差异;差异甲基化水平可能与辐射抗拒相关。

英文摘要：

Objective To expound the pathogenetic mechanism of radiation resistance of NPC from an epigenetics perspective by discussing the differences of the DNA methylation levels of nasopharyngeal carcinoma（NPC） cells in the same genetic background but different radiation resistances. Methods Me DIP-Seq was performed to sequence the CNE-2 and CNE-2R cells. The distribution of different intervals of DNA methylation level was analyzed to detect the association between the DNA methylation and radiation resistance. Results The differences mostly centralized in 5, 6, 7, 9, 13 and 17 chromosomes, which were up-regulated in 5, 6, 13 and 17 chromosomes and down-regulated in 7 and 9 chromosomes, respectively.Conclusion The DNA methylation levels between NPC cells in the same genetic background but different radiation resistances（CNE-2 and CNE-2R cells） were quite different, indicating that DNA methylation may be associated with NPC radioresistance.