全身的 annexin 基因, MtAnn3, Medicago, truncatula 被 5 种族克隆。与典型 annexins,它在保存核心领域包含一个头领域和四次相应重复相比, MtAnn3 蛋白质让仅仅在核心领域重复。当短暂地在洋葱表示了时, MtAnn3 能绑房间膜表皮的房间。进 Medicago 根的 MtAnn3 的 Agrobacterium 调停 rhizogenes 的转变表明基因的 overexpression 能在 Ca2+ 免费的媒介改变根头发生长的极性。植物荷尔蒙细胞激动素对 upregulate 有能力 MtAnn3 的表示。当 MtAnn3 抄本在年轻小瘤被检测时,表示不是小瘤特定的,并且能也在根,茎和叶子在高水平被检测。
The full-length annexin gene, MtAnn3, of Medicago truncatula was cloned by 5' RACE. Compared with typical annexins, which contain a head domain and four homologous repeats in the conserved core domain, the MtAnn3 protein has only one repeat in the core domain. MtAnn3 can bind cell membranes when transiently expressed in onion epidermal cells. Agrobacterium rhizogenes-mediated transformation of MtAnn3 into Medicago roots revealed that overexpression of the gene can change the polarity of root hair growth in Ca^2+-free medium. The plant hormone cytokinin was able to upregulate the expression of MtAnn3. While MtAnn3 transcripts were detected in young nodules, expression was not nodule-specific, and could be detected at high levels in the roots, stems and leaves as well.