中文摘要：

目的 探讨火棘多糖对四氯化碳（CCI_4）所致大鼠肝损伤的保护作用。方法 将50只Wistar大鼠随机分为5组：对照组,CCI_4模型组,联苯双酯（BP）组,火棘多糖200、400 mg/kg组。对照组大鼠中腹膜下注射橄榄油,其余各组大鼠腹膜下注射30%CCI_4,联苯双酯组、火棘多糖组分别灌胃BP 200 mg/kg,火棘多糖200、400 mg/kg。5周后,测定血清中丙氨酸转氨酶（ALT）及天冬氨酸转氨酶（AST）含量;检测肝组织中丙二醛、谷胱甘肽过氧化物酶（GSH-Px）含量及总抗氧化能力（T-AOC）活性;用real time PCR检测大鼠肝组织中核因子相关因子2（Nrf2）及过氧化氢酶（CAT）mRNA表达变化。结果 与CCI_4模型组大鼠血清ALT、AST含量[（521.42±68.21）、（589.17±39.44）U/L]比较,火棘多糖200、400 mg/kg组大鼠血清ALT、AST含量[分别为（221.13±28.46）、（190.31±27.32）与（286.4±31.57）、（224.6±30.52）U/L]明显降低,差异有统计学意义（P〈0.05）;与CCI_4模型组大鼠肝组织中丙二醛含量[（32.14±3.26）nmol/mg pro]比较,火棘多糖200、400 mg/kg组大鼠肝组织中丙二醛含量[分别为（23.29±4.38）、（18.67±3.28）nmol/mg pro]明显降低,差异有统计学意义（P〈0.05）;与CCI_4模型组大鼠肝组织中T-AOC活性[（73.15±9.23）U/m L]比较,火棘多糖200、400 mg/kg组大鼠肝组织中T-AOC活性[分别为（106.32±14.23）、（152.31±16.12）U/m L]明显升高,差异有统计学意义（P〈0.05）;real time PCR结果显示,与CCI_4模型组比较,火棘多糖组大鼠肝组织中Nrf2及CAT mRNA表达水平增加。结论 火棘多糖对CCI_4所致大鼠肝损伤具有保护作用,其机制可能与火棘多糖可提高大鼠抗氧化能力有关。

英文摘要：

Objective To study hepatoprotective effect of Pyracantha fortuneana polysaccharides on liver injury in- duced by carbon tetrachloride （ CC[4 ） in rats. Methods Fifty Wistar rats were randomly divided into five groups ： con- trol group, CCI4 group, biphenyldicarboxylate pills （BP） group, and 200 and 400 mg/kg Pyracantha fortuneana polysac- charides groups. The rats of control group were given olive oil by intraperitoneal injection （IP） and those of other groups were given 30% CCI4 by IP,respectively. The rats of BP group were given BP （200 mg/kg） by gavage and the rats of Pyracantha fortuneana polysaccharides groups were given Pyracantha fortuneana polysaccharides of 200 and 400 mg/kg by gavage, respectively. Five weeks after the treatments, the contents of serum alanine transferase （ALT） and aspartate transaminase （AST） were determined; the levels of malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） and the activity of total antioxidative capacity （T-AOC） in liver were measured. The mRNA expression levels of nuclear factor erythroid 2-related factor （Nrf2） and catalase （CAT） were detected with real time PCR. Results The serum ALT （221.13 ±28.46 and 190. 31 ±7. 32 U/L） and AST （286.4 ±1.57 and 224. 6 ±0. 52 U/L） decreased remark- ably in the rats of 200 and 400 mg/kg Pyracantha fortuneana polysaccharides groups compared to those of the rats in CCI4 group （521.42 ±68.21 and 589. 17 ±39.44 U/L） （ all P 〈 0. 05 ）. The MDA （23.29 ± 4. 38 and 18.67 ±3.28 nmol/mg pro） in liver of the rats of 200 and 400 mg/kg Pyracantha fortuneana polysacchafides groups decreased re- markably compared to that of rats in the CCI4 group （both P 〈0. 05）. The T-AOC （106. 32 ±14. 23 and 152. 31 ±16. 12 U/mL） in liver of the rats in 200 and 400 mg/kg Pyracantha fortuneana polysaccharides groups increased significantly compared to that （ 73.15 ± 9. 23 U/mL） of the rats in CCI4 group （ both P 〈 0. 05 ）. The mRNA expression levels of Nrf2 a