中文摘要：

目的探讨去势法联合皮质激素快速诱导兔骨质疏松模型椎体骨小梁Micro-CT动态变化规律。方法 40只8个月龄雌性大白兔随机分为假手术组（A组,n=20）及去势＋肌注甲强龙组（B组,n=20）。术后第2、4、8、10周行血清骨转化标志物检测及离体腰5椎体Micro-CT扫描。每组在每个时间点动物数5只。结果两组vBMD在术后第8周开始,差异有统计学意义,而BVF、Tb.N、Conn.D、Tb.Sp、SMI及Tb.Th分别在术后4、8周开始,差异有统计学意义。B组术后TRAP-5b迅速增高,至第4周达峰值,术后第4、8、10周,两组TRAP-5b差异有统计学意义;而BALP缓慢逐渐增高,至第8周才达峰值,术后第8、10周,两组BALP差异有统计学意义。结论去卵巢＋肌注甲强龙可在8周内快速建立兔OP模型,骨微结构衰败早于vBMD变化而与骨转化标志物同步。

英文摘要：

Objective To observe the morphological characteristics of osteoporosis（OP） in a overiectomized rabbit model with micro-computed tomography（micro-CT）.Methods Forty 8-month-old, skeletally mature,female New Zealand white rabbits were randomly allocated into two groups; group B（n = 20） underwent bilateral overiectomy（OVX） combined with intramuscular injection of methylprednisolone hemisuccinate（MPH）;group A（n =20） were used as sham operation controls. Five rabbits from both groups were sacrificed at 2,4,8 and 10 weeks after OVX,respectively.The serum bone metabolism markers were measured and micro-CT scanning of L5 vertebral body was performed.Results The bone mineral density（BMD） of group B at 8 and 10 weeks after OVX was significantly decreased compared to that of group A（P0.01）.The microstructural parameters BVF, Conn.D,Tb.N,SMI,Tb.Sp and Tb.Th in group B were significantly lower than those in group A at 4 and 8 weeks after OVX.Serum TRAP-5b in group B was significantly increased at 4,8 and 10 weeks after OVX compared to group A（P 0.01）;while serum BALP was significantly increased at 8 and 10 weeks（P 0.01）.Conclusion Osteoporosis can be induced in rabbits by combining overiectomy with corticosteroid administration in 8 weeks.The deterioration of trabecular microarchitecture occurs earlier than that of vBMD,but in synchrony with changes of bone turnover markers.