中文摘要：

目的：观察健脾益肺化痰系列方对慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）模型大鼠的防治作用及其机制。方法：采用单纯香烟烟熏法建立COPD大鼠模型。将60只WiStar雄性大鼠随机分为正常组、模型组、健脾益肺化痰方组、健脾益肺方组、化痰方组和羧甲司坦组，每组10只。健脾益肺化痰方组、健脾益肺方组、化痰方组和羧甲司坦组大鼠分别给予相应的药物灌胃治疗，正常组和模型组灌胃等体积纯净水。用酶联免疫测定法（ELISA）检测各组大鼠肺泡灌洗液（BALF）中黏蛋白5AC（MUC5AC）、黏蛋白5B（MUC5B）、白细胞介素1β（IL-1β）、环氧化酶2（COX2）、转化生长因子α（TGF—α）、前列腺素E2（PGE2）的含量。结果：与正常组比较，模型组大鼠肺泡灌洗液（BALF）中MUC5AC、MUCSB、IL-1β、COX2、TGF-α、PGE2含量显著升高（P〈0．05）。与模型组比较，健脾益肺化痰组、健脾益肺组、化痰组大鼠肺泡灌洗液（BALF）中MUC5AC、MUC5B、IL-1β、COX2、TGF—α、PGE2含量均降低（P〈0．05）。结论：健脾益肺化痰系列方中以标本兼治的健脾益肺化痰方尤为突出，能降低COPO模型大鼠肺泡灌洗液中MUC5AC、MUC5B、IL-1β、COX2、TGF-α、PGE2的含量，抑制气道黏液高分泌传导通路，使气道结构重塑，改善气道通气状况，最终改善COPD模型大鼠肺功能。

英文摘要：

Objective： To observe the preventive, therapeutic effect and mechanism of series of prescriptions of tonifying the spleen and nourishing the lung as well as eliminating phlegm on the rat model of chronic obstructive pulmonary disease. Methods： Rat model with COPD was established by method of simple cigarette smoking. 60 male Wistar rats were randomly divided into normal control group, model group, tonifying the spleen and nourishing the lung as well as eliminating phlegm decoction group, tonifying the spleen and nourishing the lung decoction group, eliminating phlegm decoction group and carbocysteine group, ten rats in each group. Rats in tonifying the spleen and nourishing the lung as well as eliminating phlegm decoction group, tonifying the spleen and nourishing the lung decoction group, eliminating phlegm decoction group and carbocysteine group were given corresponding drugs by gavage. At the same time, the normal group and model group were perfused with the same volume of pure water. Enzyme linked immunosorbent assay （ELISA） was used to detect the contents of mucoprotein 5AC （MUC5AC）, mucoprotein 5B（MUC5B）, interleukin 1 beta（IL-1β）, cyclooxygenase 2（COX2）, transforming growth factor alpha（TGF-a） and prostaglandin E2（PGE2） in bronchoalveolar lavage fluid of rats in each group. Results： Compared to the normal group, MUC5AC, MUC5B, COX2, IL-1β, TGF-α, and PGE2 in the bronchoalveolar lavage fluid （BALF） of model group were significantly increased （P〈0.05）. While compared to the model group, MUC5AC, MUC5B, COX2, IL-1β, TGF-α, and PGE2 in the bronchoalveolar lavage fluid （BALF） of tonifying the spleen and nourishing the lung as well as eliminating phlegm decoction group, tonifying the spleen and nourishing the lung Decoction group and eliminating phlegm decoction group were all decreased （P〈0.05）. Conclusion： It is outstandingly true of the tonifying the spleen and nourishing the lung as well as eliminating phlegm decoction which can treat both principal