中文摘要：

使用简并PCR结合RACE技术从能源植物小桐子种子中克隆了一个PDAT基因的cDNA全长,命名为JcPDAT1（GenBank登陆号为HQ827796）。JcPDAT1全长2869bp,包含一个长为2013bp的开放阅读框,编码670个氨基酸。多序列比对和进化分析表明该基因编码蛋白与其他植物PDAT高度同源,具有典型的PDAT结构域。Realtime PCR结果表明JcPDAT1在种子、叶和根里面都有表达,且在种子发育过程中大量表达。酵母互补实验证实该基因编码蛋白具有PDAT酶活性。在与酿酒酵母突变体H1246α的互补实验中发现,TLC层析（薄层层析法）和尼罗红染色结果都显示JcPDAT1的表达使H1246α恢复合成TAG,说明JcPDAT1具有PDAT的功能活性。

英文摘要：

According to the conserved region of PDAT gene available from GenBank, the cDNA sequence of PDAT gene from Jatropha curcas L. was cloned by degenerative PCR and RACE. The gene was named JcPDAT1 （GenBank accession HQ827796）. The full length cDNA of JcPDAT1 is 2 869bp, encoding 670 amino acids. Sequence alignment and phylogenetic analysis revealed that JcPDAT1 shared high homology with PDAT from other plants, such as RcPDAT1 （Ricinus communis, 75%） and AtPDAT1 （Arabidopsis thaliana, 74%）. The sequence harbored typical functional domains of PDAT. Realtime PCR showed that JcPDAT1 was expressed in different tissues including seeds, leaf, root tip, and was highly expressed in developing seeds, especially at the oil accumulation stage. TLC analysis and Nile red staining showed that JcPDAT1 could restore the TAG biosynthesis and accumulation in Saccharomyces cerevisiae mutant strain H1246？？？which indicated that the JcPDAT1 possessed PDAT enzyme activity.