中文摘要：

目的探讨人巨细胞病毒（HCMV AD169株）感染对CHRF原巨核细胞成活率的影响及其机制。方法采用CHRF细胞培养技术，利用HCMV原液直接感染CHRF细胞株；用RT-PCR观察鉴定HCMV是否能直接感染CHRF细胞株：用MTT法检测细胞存活率；流式细胞仪分析HCMV感染后诱导细胞凋亡发生率；免疫印迹分析caspase-3活性。结果①HCMV AD169能直接感染CHRF细胞：②HCMV AD169感染浓度和时间依赖性地降低CHRF细胞成活率；③HCMV AD169诱导CHRF细胞发生凋亡及caspase-3的激活。结论体外感染HCMV通过抑制增殖和诱导凋亡降低CHRF细胞的成活率。这些结果有助于理解HCMV感染引起血小板减少症的病理机制。

英文摘要：

Objective To investigate the effects and action mechanisms of human cytomegalovirus （HCMV AD169） on viability of CHRF megakaryoblastic cells. Methods After CHRF cells were exposed to HCMV AD169, RT-PCR was used to detect whether HCMV directly infected CHRF; MTT, flow cytometry and western blot assays were used to determine the viability of cells, apoptotic rates and the activated caspase-3, respectively. Results ①CHRF cells were permissive for HCMV AD169 infection; ②HCMV AD169 dose- and time-dependently reduced the viability of CHRF cells; and ③HCMV AD169 caused apoptosis and the activation of caspase-3 in CHRF cells. Collusion The infection of HCMV may reduce viability of megakaryoblastic cells through proliferation inhibition and apoptosis, which may be involved in the pathogenesis of HCMV-associated thrombecytopenia.