由瘤胃原生动物的细菌的捕食率上的研究用标记荧光的细菌(FLB ) 的一种技术在实验室条件下面被执行。四只 Xuhuai 山羊在这个实验被使用获得瘤胃原生动物和细菌。二个组如下被设计:一个组是通过把免费细菌从瘤胃液体(WFLB ) 移开用荧光被标记的整个细菌;另外的组是没有把免费细菌从瘤胃液体(FLB ) 移开,用荧光被标记的细菌。结果显示细菌的捕食瘤胃原生动物评价为组 WFLB 是 398.4 cells/(房间 h ),为组 FLB 的 230.4 cells/(房间 h )当相应价值表示了为 bacteria-N 时,他们为组 WFLB 是 2.15 pg N/(房间 h ),并且为组 FLB 的 1.24 pg N/(房间 h )分别地。外推由 Xuhuai 山羊的细菌上的纤毛虫类的氮的吸收数量,分别地为组 FLB 为组 WFLB,和 59.5 mg N/(d 人) 有 103.2 mg N/(d 人) 。蛋白质损失分别地为组 FLB 为组 WFLB 和 0.372 g pro/(d 人) 由于微生物引起的再循环是 0.645 g pro/(d 人) ,这被估计。另外,标记荧光的技术将是为由瘤胃原生动物的细菌的捕食率的决心的潜在的试金。
Studies on the bacterial predation rate by rumen protozoa were carried out under laboratory conditions using a technique of fluorescence-labeled bacteria (FLB). Four Xuhuai goats were used in this experiment to obtain rumen protozoa and bacteria. Two groups were designed as follows: One group was the whole bacteria which were labeled using fluorescence through removing free bacteria from rumen fluid (WFLB); the other group was the bacteria which were labeled using fluorescence without removing free bacteria from rumen fluid (FLB). The result indicated that the bacterial predation rates of rumen protozoa was 398.4 cells/(cell h) for the group WFLB, 230.4 cells/(cell h) for the group FLB, when the corresponding values expressed as bacteria-N, they were 2.15 pg N/(cell h) for the group WFLB, and 1.24 pg N/(cell h) for the group FLB, respectively. Extrapolating the assimilation quantity of nitrogen by ciliates on bacteria of Xuhuai goat, there were 103.2 mg N/(d capita) for the group WFLB, and 59.5 mg N/(d capita) for the group FLB, respectively. It was estimated that protein losses due to microbial recycling were 0.645 g pro/(d capita) for the group WFLB and 0.372 g pro/(d capita) for the group FLB, respectively. In addition, the fluorescence-labeled technique would be a potential assay for the determination of bacterial predation rate by rumen protozoa.