中文摘要：

建立了快速测定水产加工食品中河豚毒素（tetrodotoxin，TTX）的超高效液相色谱-串联质谱（UPLC-MS／MS）分析方法。样品经0．1％乙酸水溶液提取，C18萃取剂分散固相萃取净化，超滤管过滤后进行分析。目标物TYX采用TSK-gelAmide-80亲水色谱柱进行分离，以电喷雾离子源、多反应监测（MRM）正离子模式检测，基质标准曲线校正，外标法定量。结果表明，TTX在2-200ng／mL范围内线性良好（r^2〉0．999），方法的定量限为10μg/kg，加标回收率为71．2％～102％，相对标准偏差（RSD）为5．23％-12．4％。方法操作简便，准确度和精密度良好，可用于烤鱼片、鱼干等干制水产加工食品中河豚毒素的测定，具有较好的应用价值。

英文摘要：

A method for the rapid determination of tetrodotoxin （TTX） in aquatic food using ultra performance liquid chromatography- tandem mass spectrometry（ UPLC -MS/MS） has been established. TTX was extracted from sample by 0.1% acetic acid -water, and then purified by dispersive solid -phase extraction with Cls extraction sorbent and filtered through a Mr 10000 cut - off ultrafree microcentrifuge filter. The separation of TYX was carried out on a col- umn of TSK - gel Amide - 80, and detected by positive electrospray ionization tandem mass spectrometry in the MRM mode. The matrix- matched external standard calibration curves were used for quantitative analysis. The calibration curves were linear in the range of 2 - 200 ng/mL for TI＇X, with quantification limit of 10 μg/kg. The average recover- ies were between 71.2% and 102% with relative standard deviations（RSD） ranging from 5.23% to 12.4%. The proposed method was simple, rapid, accurate and applicable for the determination of TTX in aquatic food.