中文摘要：

目的对腺相关病毒（adeno-associated virus，AAV）Rep78蛋白对乙肝病毒X启动子（HBV—X promoter）的可能作用进行初步研究。方法利用pMAL-Rep78（含有Rep78全长基因的表达质粒）在大肠杆菌中表达并纯化得到带有AAVRep78蛋白的MBP—rep78融合蛋白；通过SDS-电泳及免疫印迹（Western blot）鉴定蛋白表达。合成地高辛标记的X启动子3段探针；以非放射性凝胶电泳阻滞实验（EMSA）检测Rep78与HBV-X启动子的结合。结果得到纯化的MBP—rep78融合蛋白；EMSA结果显示Rep78蛋白在体外能够与HBV—X启动子DNA结合，并有剂量依赖关系。结论AAVRep78可以与HBV—X启动子结合。

英文摘要：

Objective This study is to investigate the possible effect of adeno-associated virus （AAV） Rep78 on hepatitis B virus X gene （HBV-X）. Methods Rep78 protein was produced as a fusion protein with MBP using pMAL-Rep78 plasmid （with full gene of Rep78） in DH-5α. SDS-PAGE and western blot were used to confirm the expression of MBP-Rep78. Electrophoretic mobility shift assay（EMSA） was utilized to detect the binding of MBP-Rep78 protein with HBV-X promoter. Results Purified MBP-Rep78 fusion protein was obtained successfully. EMSA showed Rep78 protein could bind with HBV-X promoter strongly in a dose-dependent manner. Conclusion AAV Rep78 could bind with HBV-X promoter.