中文摘要：

利用高效液相色谱法对肉鸭肝脏中参与脂肪酸合成关键酶——脂肪酸合成酶的活性进行分析,并测定了鱼腥草素对鸭肝脂肪酸合成酶活性的影响。采用离心法从鸭肝中分离纯化脂肪酸合成酶,并用鱼腥草素（100 mg/L）处理脂肪酸合成酶,然后用HPLC法检测还原型NADPH在340 nm处吸收峰变化。HPLC最佳测定条件为：Hypersil C18色谱柱,流动相：0.01 mol/L磷酸盐缓冲液（pH7.2）-甲醇溶液（80∶20,V/V）,柱温：25℃,流速：1 mL/min,检测波长：340 nm,进样量：20μL。HPLC加样的回归方程：A=17754x＋123.3,r=0.9945。实验结果表明,还原型烟酰胺腺嘌呤二核苷酸（NADPH）浓度在0.5μmol/L～1.44 mmol/L内线性关系良好。HPLC法检测鸭肝脂肪酸合成酶的活性,样品用量少,准确度高。

英文摘要：

A high performance liquid chromatography method measuring the fatty acid synthase （FAS） activity and the effect of HOU-Cn to FAS activity were established. FAS from duck liver was treated with houttuyfonate homologues （ HOU-Cn ）. Then the change in the activity of FAS was determined by HPLC. The optimal conditions of HPLC were as follows： Hypersil C18 column, mobile phase： 0.01 mol/L phosphate buffer （pH 7.2）- methanol （80：20, v/v）, column temperature： 25 ℃, flow rate： 1 mL/min, detection wavelength： 340 nm, injection volume ： 20 μL. The regression equation of HPLC was ： A = 17754x ＋ 123.3, r = 0.9945. An excellent linearity over the range of 0.5 μmol/L - 1.44 mmol/L was observed. The HPLC method can be used to determine the FAS activity with less dosage and high accuracy.