中文摘要：

摘要：为了探索IGF-1基因的启动子结构特征对猪体长性状的影响，应用PCR方法从藏猪、巴马小型猪、野猪、军牧一号猪的基因组DNA中分别克隆测序了IGF-1基因启动子区序列，并结合生物信息学手段分析了不同品种猪之间启动子区转录因子结合位点数目和序列特征的差异，发现在IGF-1基因启动子区，巴马小型猪与藏猪、东北野猪、军牧一号猪相比，缺失CdxA、Nkx-22个转录因子结合位点，但比藏猪、东北野猪多了1个MZFl转录因子结合位点；藏猪、东北野猪与军牧一号猪相比，缺失1个MZFl转录因子结合位点，邻接法构建的分子进化树发现，藏猪与巴马小型猪亲缘关系最近，与东北野猪亲缘关系相对较近，但与军牧一号猪亲缘关系最远。结果表明：猪的IGF-1基因启动子结构在不同体长猪种上存在一定差异。

英文摘要：

In order to explore the structural characteristics of IGF- 1 gene promoter for long character in pigs, the IGF- 1 gene 5＇UTR was amplified and sequenced from the genomic DNA in four different pig breeds： Tibet Pig, Bama miniature Pig, Northeastern Wild boar and Junmu- 1 Pig. With bioinformatics tools, characterization and comparison of these sequences from four varieties of swine were conducted. Results showed that： In IGF- 1 gene 5＇ end, compared with the others, Bama miniature Pig missed a possible CdxA binding site and a possible NKx - 2 binding site. Moreover, comparing Junmu - 1 Pig with Tibet Pig and Northeastern Wild boar, we found a possible MZF1 binding site in Junmu - 1 Pig. The evolutionary tree among four species, constructed by the neighbor - joining method, showed that Tibet Pig had a recently genetic relationship with Bama miniature pig; and a closely genetic relationship with Northeastern Wild boar. And the genetic relationship between Junmu- 1 Pig and the others was the far- thest. The resuhs show that the structure of IGF- 1 gene promoter is different in different body length pigs.