中文摘要：

Angiogenin 是涉及 tumorigenesis 的一个 angiogenic 因素。然而， angiogenins 行动的机制留下逃犯。在现在的学习，我们识别了 fibulin 1，细胞外的矩阵和血浆 glycoprotein，由酵母的一个交往 angiogenin 分子二混血儿的屏蔽。这个相互作用被二条不同途径进一步证实。首先， fibulin 1 是有由在 vitro 的 anti-angiogenin monoclonal 抗体的 angiogenin 的 co-immunoprecipitated，建议 angiogenin 与 fibulin 绑 1 直接。然后，荧光回声精力转移分析显示出那 fibulin 1 在 COS-7 房间与 angiogenin 交往了，证明绑定能发生在细胞的上下文。作为 fibulin， 1 在房间增长起一个重要作用，移植，粘附，并且稳定新形成的血容器墙，在 fibulin 之间的相互作用 1 并且 angiogenin 可能在 angiogenesis 或 tumorigenesis 加重 angiogenin 的一可能的机制。

英文摘要：

Angiogenin is an angiogenic factor involved in tumorigenesis. However, the mechanism of angiogenin＇s action remains elusive. In the present study, we identified fibulin 1, an extracellular matrix and plasma glycoprotein, as an angiogenininteracting molecule by yeast two-hybrid screening. This interaction was further confirmed by two different with angiogenin by anti-angiogenin monoclonal antibody in vitro, suggesting angiogenin binds with fibulin 1 directly. Then fluorescence resonance energy transfer analysis showed that fibulin 1 interacted with angiogenin in COS-7 cells, showing that the binding could occur in a cellular context. As fibulin 1 plays an important role in cell proliferation, migration, adhesion, and stabilizes new-forming blood vessel wall, the interaction between fibulin 1 and angiogenin might underline one possible mechanism of angiogenin in angiogenesis and/or tumorigenesis.