中文摘要：

目的观察2型糖尿病（T2DM）及其微血管并发症（T2DMC）患者血清miR-661水平变化并分析其临床辅助诊断价值。方法用实时荧光定量PCR技术（qRT-PCR）检测60例T2DM患者、60例T2DMC患者及60例健康对照者血清中miR-661水平,同时测定空腹血糖（FPG）、总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）和糖化血红蛋白（HbA1c）等指标水平,ROC曲线、相关性分析及Logistic regression分析血清miR-661对T2DM及T2DMC的诊断价值。结果qRT-PCR结果显示,T2DM患者和T2DMC患者血清miR-661相对含量[中位数（四分位数）][M（P25,P75）]分别为230.1（83.6,426.2）fmol/L和441.3（212.9,1 021.0）fmol/L,与健康人对照组的118（63.6,192.4）fmol/L相比,均升高（U值分别为1 207和435,P均〈0.01）;血清miR-661 T2DM的ROC曲线下面积（AUC~（ROC））为0.665（95%CI=0.565~0.765,P〈0.01）,T2DMC的AUC~（ROC）为0.879（95%CI=0.821~0.938,P〈0.01）,区分T2DM及T2DMC的AUC~（ROC）为0.694（95%CI=0.600~0.788,P〈0.01）;相关性分析结果显示血清miR-661水平与FPG和LDL-C呈正相关（r值分别为0.291和0.149,P均〈0.05）,与HDL-C呈负相关（r为-0.243,P〈0.01）;进一步用单因素和多因素Logistic regression分析结果显示,血清miR-661是T2DM和T2DMC的独立危险因素（P均〈0.01）。结论血清中表达升高的miR-661是T2DM及T2DMC患者潜在辅助诊断标志物及独立危险因素。

英文摘要：

Objective To investigate the serum levels of miR-661 in type 2 diabetes （T2DM） patients and type 2 diabetes with microvascular complications （T2DMC）,and to further evaluate its clinical auxiliary diagnosis significance.Methods The serum levels of miR-661 were examined in 60 T2DM patients,60 T2DMC patients and 60 healthy controls using quantitative real-time PCR （qRTPCR）.The sera levels of biochemical parameters including fasting plasma glucose （FPG）,cholesterol （TC）,triglycerides （TG）,low density lipoprotein （LDL-C）,high density lipoprotein （HDL-C） and haemoglobin A1C （HbA1c） were determined by biochemical analyzer.The ROC curve analysis,correlation analyses and logistic regression analyses were performed to evaluate the clinical auxiliary diagnosis value of serum miR-661,respectively.Results The qRT-PCR results showed that the concentrations of miR-661 was 118 （63.6,192.4） fmol/L in healthy controls,which were significant lower than those of T2DM patients[230.1 （83.6,426.2） fmol/L,（U= 1 207,P〈0.01） ]and T2DMC patients[441.3 （212.9,1 021） fmol/L,（U = 435,P〈0.01） ].ROC curve （AUC~（ROC）） of miR-661 was 0.665 （95% CI ： 0.585 ~ 0.765,P〈0.01） for T2DM patients and 0.879 （95% CI： 0.821 ~ 0.938,P〈0.01） for T2DMC patients.The AUC~（ROC）between T2DM and T2DMC patients was 0.694 （95% CI： 0.600 ~ 0.788,P〈0.01）.The correlation analyses revealed that the levels of miR-661 was positively correlated with concentrations of FPG （r = 0.291,P〈0.05） and LDL-C （r = 0.149,P〈0.05）,and negatively correlated with concentrations of HDL-C （r =-0.243,P〈0.01）.Logistic regression analysis revealed that serum miR-661 was an independent risk factor in T2DM and T2DMC （P〈0.01）.Conclusion Serum miR-661 was potential biomarker and risk factor for T2DM and T2DMC.