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基于原子力显微镜微切削加工技术的癌细胞内部弹性模量计算
  • ISSN号:0023-074X
  • 期刊名称:科学通报
  • 时间:2012
  • 页码:3533-3537
  • 分类:Q26[生物学—细胞生物学] TH742[机械工程—光学工程;机械工程—仪器科学与技术;机械工程—精密仪器及机械]
  • 作者机构:[1]State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150001, China, [2]Center for Precision Engineering, Harbin Institute of Technology, Harbin 150001, China, [3]The Fourth Department of Neurosurgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
  • 相关基金:This work was supported by the National Natural Science Foundation of China (51175124) and the Self-Planned Task of State Key Laboratory of Robotics and System of Harbin Institute of Technology (SKLRS 200903C).
  • 相关项目:基于原子力显微镜生物组织的纳米加工检测一体化技术的研究
中文摘要:

在癌症房间的机械性质的变化的更好的理解能帮助为癌症的诊断,预防,和治疗提供新奇答案。我们因此基于在一台原子力量显微镜和目标的硅尖端之间的接触压力开发了细胞内部的有弹性的模量的一个计算模型房间,和切的深度。卵巢的房间(UACC-1598 ) 和结肠癌房间(NCI-H716 ) 用一个原子力量显微镜硅尖端被切成顺序的层。当 Ge 内容更慢慢地增加时,房间上的切的区域是 8 m。根据试验性的数据,在 Ge 内容(x) 和 F (GeH4 ) 之间的一种新关系 /F (SiH2Cl2 ) 质量流动比率被推出:x 2.5/(1x )= nF (GeH4 )/F (SiH2Cl2 ) 。SiGe 生长率和 Ge 内容与增加反应堆房间压力改善。由选择合适的先锋流动和反应堆压力,有一样的 Ge 的 SiGe 电影满足能在各种各样的温度被制作。然而, SiGe 晶体的质量清楚地依赖于免职温度。在更低的免职温度,更高水晶的质量被完成。因为生长率戏剧性地与更低的温度落下,最佳生长温度一定是在水晶的质量和生长率之间的妥协。X 光检查衍射,拉曼散布光谱学和原子?

英文摘要:

Better understanding of variations in the mechanical properties of cancer cells could help to provide novel solutions for the diagnosis, prevention, and treatment of cancers. We therefore developed a calculation model of the intracellular elastic modulus based on the contact pressure between the silicon tip of an atomic force microscope and the target cells, and cutting depth. Ovarian cells (UACC-1598) and colon cancer cells (NCI-H716) were cut into sequential layers using an atomic force microscope silicon tip. The cutting area on the cells was 8 ~tm x 8 ~tm, and the loading force acting on the cells was increased from 17.523 to 32.126 μN. The elastic modulus distribution was measured after each cutting process. There were significant differences in contact pressure and cutting depth between different cells under the same loading force, which could be attributed to differences in their intrinsic structures and mechanical properties. The differences between the average elastic modulus and surface elastic modulus for UACC-1598 and NCI-H716 cells were 0.288±0.08 kPa and 0.376±0.16 kPa, respectively. These results demonstrate that this micro-cutting method can be used to measure intracellular mechanical properties, which could in turn provide a more accurate experimental basis for the development of novel methods for the diagnosis and treatment of various diseases.

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期刊信息
  • 《科学通报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院
  • 主编:周光召
  • 地址:北京东黄城根北街16号
  • 邮编:100717
  • 邮箱:csb@scichina.org
  • 电话:010-64036120 64012686
  • 国际标准刊号:ISSN:0023-074X
  • 国内统一刊号:ISSN:11-1784/N
  • 邮发代号:80-213
  • 获奖情况:
  • 首届国家期刊奖,中国期刊方阵“双高”期刊,第三届中国出版政府奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),美国数学评论(网络版),美国工程索引,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:81792