中文摘要：

在大肠杆菌Rosetta（DE3）中表达猪瘟病毒石门株NS3基因部分功能片段，以纯化的重组NS3（p80）蛋白免疫BALB／c小鼠，取其脾细胞与骨髓瘤细胞SP2／0进行融合，经间接ELISA筛选及3次连续克隆化获得了4株可分泌抗NS3单抗的杂交瘤细胞（2G7，2F11，5D2和6F11），通过小鼠体内诱生法制备腹水，并对杂交瘤细胞和单克隆抗体的特性进行鉴定。结果表明，杂交瘤细胞染色体正常，抗体分泌稳定，单抗效价高，亲和力各不相同，Ig亚类鉴定均为IgGl。Western blot分析证明4株单抗均能与原核表达的NS3蛋白特异性结合，6F11株与真核表达的NS3蛋白有较强的特异性反应。

英文摘要：

Monoclonal antibodies against NS3 gene of CSFV was developed using recombinant NS3 （p80） protein expressed in E.coli Rosetta （DE3） as antigen. BALB/c mice was immunized with purified NS3 （pS0） protein and mouse splenic cells were fused with SP2/0 cells. Hybridoma cells were screened by ELISA, subcloned and characterized. Four hybridoma cells （2G7, 2F11, 5D2 and 6F11） secreting anti-NS3 monoclonal antibodies （McAbs） were obtained. Hybridoma cells with normal chromosome were shown to stably secret NS3 specific antibodies with various affinities. All the McAbs belong to IgG1 subtype.