中文摘要：

目的探讨大鼠深静脉血栓（DVT）模型股静脉内皮组织中整合素基因Itgct。（integrinalpha5）和Itgl3，（integrinbeta3）的表达变化及其在血栓形成中的作用。方法将60只SD大鼠随机分为对照组（10只）和模型组（50只），对模型组采用股静脉钳夹联合双下肢石膏制动构建大鼠DVT模型。不同时问点（造模后2．5h和25h）解剖股静脉、观测血栓的发生率，进而将模型组分为：血栓形成前组（造模后2．5h）、血栓形成组（造模后25h）、血栓不形成组（造模后25h）。分离股静脉内皮组织，提取总RNA；采用GenechipRatGenome2302．0基因芯片筛查差异表达的基因；采用实时荧光定量聚合酶链式反应（real—timePCR）验证这些基因的表达变化。结果基因芯片分析及real—timePCR结果均发现：大鼠股静脉组织中Itgct。貌和Itsl3，的表达水平在血栓形成组最高，血栓形成前组次之，均明显高于对照组和血栓不形成组（P〈0．05）。结论大鼠股静脉内皮组织中Itgct，烷和ItN3，表达水平上调可能在深静脉血栓形成中发挥了重要作用。

英文摘要：

Objective To study the underlying role of Itgα V （integrin alpha V ） and Itgβ3 （integrin beta 3 ） in rat deep vein thrombosis （DVT） model. Methods 60 SD rats were randomly divided into control group （controlled blank, n = 10）, pre - thrombogenesis group （2.5 hours after model built）, thrombogenesis group （25 hours after model built） and non -thrombogenesis group （25 hours after model built）. DVT rat model was established by clamping both femoral veins in combination with cast fixing. The incidence and severity degrees of thrombus were observed by dissecting rat femoral veins in different time points. Then total RNA and proteins were extracted from the localized femoral venous endothelial tissues. After gene chip - based screen, the gene expressions of Itgcty and Itg{33 were further identified by real - time PCR （ real - time quantitative polymerase chain reaction）. Results The results of gene chip hybridization analysis and real - time PCR found that the mRNA expressions of Itgot, and Itgβ3 in rat femoral vein wall tissue were significantly up - regulated at 2.5 hours after model built （ pre - thrombogenesis group was higher than control group） （ P 〈 0.05 ）, and continued up - regulating in thrombogenesis （thrombogenesis group was higher than pre -thrombogenesis group and non- thrombogenesis group） （ P 〈 0. 05 ）. Conclusion The results from present study indicated that increased expressions of Itgav and Itgβ3 in local femoral venous endothelial tissue may play a crucial role in DVT.