中文摘要：

目的探索活性维生素D2对维生素D受体（VDR）不同表达水平的糖尿病肾病大鼠模型的保护作用及可能机制。方法腹腔注射链脲佐菌素成功建立sD大鼠糖尿病肾病模型，将建模成功的36只大鼠随机分为3组（每组12只）：VDR过表达组（肾脏原位注射含有VDR的慢病毒）、正常表达组（注射单纯载体）和低表达组（注射干扰VDR表达的慢病毒）。在此基础上分别给予每组骨化三醇（即活性维生素D3，VD，0．03μg·kg-1·d-1）治疗或花生油对照处理后，分为6组：VDR过表达＋VD组、VDR过表达＋花生油组、VDR干扰＋VD组、VDR干扰＋花生油组、空载体＋VD组、空载体＋花生油组。37d后采集标本，以实时聚合酶链反应（RT．PCR）及免疫组化方法检测大鼠肾脏组织VDR表达，并进一步检测各组转化生长因子β1（TGF-β1）、人第10号染色体缺失的磷酸酶及张力蛋白同源基因（PTEN）和胶原蛋白I（COL—1）的表达情况。采用HE及Masson染色对大鼠肾脏形态结构及纤维化程度进行分析。采用单因素方差分析（ANOVA）及t检验法比较各组数据。结果RT．PCR及免疫组化结果显示，与VDR过表达＋花生油组相比，VDR过表达＋VD组VDR和PTENmRNA及蛋白表达均升高（t=-19．67、-5．18、-25．57、-10．06，均P〈O．01）；与VDR过表达＋花生油组相比，VDR过表达＋VD组TGF．131和COL-1mRNA及蛋白表达均降低（t=15．62、8．20、28．28、5．81，均P〈0．01）；与VDR干扰＋花生油组相比，VDR干扰＋VD组VDR和FFENmRNA及蛋白表达量均升高（t=53．67、-7．00、-19．81、-8．06，均P〈O．01）；与VDR干扰＋花生油组相比，VDR干扰＋VD组TGF．[31和COL-1mRNA及蛋白表达量均降低（t=12．0、8．50、21．22、5．42，均P〈0．01）；与空载体＋花生油组相比，空载体＋VD组VDR和PTENmRNA及蛋白表达均升高（t=-20．39、-5．46、-25．92、-6．38，均P〈0．01）；与?

英文摘要：

Objective To explore the effects and mechanism of 1,25-dihydroxyvitamin D3 on the diabetic nephropathy with different levels of vitamin D receptor （VDR） expression in rats. Methods The models of diabetic nephropathy SD rats （n=36） were established by intraperitoneal injection of streptozotoein, then the rat models were divided into 3 groups： VDR over-expression group （kidney injection of VDR virus, n=12）, VDR normal expression group （kidney injection of viral vector, n=12） and VDR low expression group （kidney injection of VDR interfered virus, n=12）. Then the rats in the three groups were given calcitriol （0.03 μg. kg-1· d-1） or peanut oil for 37 days, six rats with one treatment in each group. So therats were divided into 6 groups： VDR over-expression＋VD group, VDR over-expression＋peanut oil group, VDR low expression＋VD group, VDR low expression＋peanut oil group, VDR normal expression＋VD group, VDR normal expression ＋ peanut oil group. The real time-polymerase chain reaction （RT-PCR） and immunohistochemistry staining were used to detect the mRNA and protein levels of VDR, transforming growth factor（TGF）-β1, phosphatase and tensin homolog deleted on chromosome ten （PTEN）, cologen-1 （COL- 1） in kidney. And the HE and Masson staining were used to detect changes of structure and the level of fibrosis in kidney. Data were compared by using t-test and one-way analysis of variance. Results The expressions of VDR and PTEN mRNA and protein in VDR over-expression ＋ VD group were significantly higher than those in VDR over-expression＋peanut oil group （t=- 19.67, -5.18, -25.57, -10.06, all P〈0.01）. The expressions of TGF-[31 and COL-1 mRNA and protein in VDR over-expression＋VD group significantly decreased than those in VDR over-expression＋peanut oil group（t=15.62, 8.20, 28.28, 5.81, all P〈0.01）. The expressions of VDR and PTEN mRNA and protein in VDR low-expression ＋ VD group were significantly higher than those in VDR low-expression ＋ peanu