中文摘要：

从一套由92R137（普通小麦-簇毛麦T6VS·6AL染色体易位系）和辉县红（地方小麦品种）杂交及以单粒传方法构建的F8重组近交家系（RIL）群体中,筛选出4个HMW-GS亚基组合相同而蛋白质含量差异较大的代表性家系（含和不含染色体易位片段的家系各一组）,采用SDS-PAGE电泳和切胶比色的亚基定量方法,研究了不同家系小麦籽粒灌浆期间HMW-GS和GMP含量动态。结果表明,小麦籽粒各亚基在花后13d均已形成,但不同亚基起始形成时间不同;各亚基含量随灌浆进程呈上升趋势,花后23d到成熟期为快速积累期。染色体易位片段对籽粒HMW-GS和GMP含量与积累量无显著作用,而籽粒HMW-GS含量以蛋白含量高的家系高于对应的低蛋白家系。

英文摘要：

Four lines from an F8 recombinant inbred line （RIL） population were identified and classed into the the T6VS·6AL and non the T6VS·6AL groups. The grain protein contents differed between the groups. The time-course changes in content and accumulation of HMW-GS and GMP during grain filling were detected. All HMW-glutenin subunits were detected at 13 d after anthesis （DAA） in grains of the four lines. However, the initial formation time differed within subunits. The content and accumulation of the HMW-GS increased during the whole grain filling period, and the rapid accumulation occurred from 23 DAA to maturity. The content and accumulation of HMW-GS and GMP showed no significant differences between the two groups. However, content and accumulation of HMW-GS of the high grain protein content lines were higher than those of the contrasting low protein content lines. In addition, grain gliadin content was found higher in the lines containing the T6VS·6AL chromosome translocation segment than in the contrasting lines.