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中文摘要：
本课题拟开展芯片毛细管电泳电化学酶联免疫分析法研究及其在植物病毒学检测技术中的应用。以HRP为标记酶，建立测定HRP的芯片毛细管电泳电化学酶联免疫分析新方法，系统地研究芯片毛细管电泳电化学酶联免疫分析新体系的酶催化反应机理及电极反应过程，丰富和发展芯片毛细管电泳电化学酶联免疫分析法的理论。将酶联免疫反应、分离、检测集成在芯片上，构建集成化的芯片毛细管电泳电化学酶联免疫分析技术平台，建立芯片毛细管电泳电化学酶联免疫分析对黄瓜花叶病毒 (CMV)、大豆花叶病毒 (SMV)、葡萄扇叶病毒(GFV)、玉米细菌性枯萎菌 (ES)等植物病毒的快速在线检测新方法，并研制相应的芯片毛细管电泳电化学酶联免疫分析检测试剂盒，以期在口岸和产地检验检疫等领域得到应用。
结论摘要：
英文主题词microchip-based capillary electrophoresis; electrochemistry; enzyme-linked immunoassay; plant virus; HRP
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期刊论文
Chronocoulometric Detection of DNA Hybridization Using reporter DNA Probe Modified with Au Nanoparti
Determination of ascorbic acid in individual rat hepatocyte cells based on capillary electrophoresis
Rapid and sensitive DNA target detection using enzyme amplified electrochemical detection based on m
Highly enhanced electrochemiluminescence of novel gold/silica/CdSe-CdS nanostructures for ultrasensi
Measurement of ascorbic acid in individual rat hepatocyte by capillary electrophoresis with electroc
Capillary Electrophoresis enzyme immunoassay for α-fetoprotein and thyroxine in human serum with ele
Ultrasensitive Flow Injection Chemiluminescence Detection of DNA Hybridization Using signal DNA prob
Determination of Physiological Thiols by Electrochemical Detection with Piazselenole and Its Applica
A Dicopper Complex Chemiluminescence Probe for the Determination of Thiols in Murine P388 Lymphocyti
Multilayers Enzyme-Coated Carbon Nanotubes as Biolabel for Ultrasensitive Chemiluminescence I mmunoa
Ultrasensitive Enhanced Chemiluminescence Determination of α-fetoprotein Amplified by Double-Codifie
Microchip-based capillary electrophoresis enzyme immunoassay for carcinoembryonic antigen and alpha-
Online Preconcentration and Two-Dimensional Separation of Cationic Compounds via Hyphenation of Capi
Online purification and determination of B-antagonists in blood sample based on heart-cutting capill
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