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信号肽介导亚细胞区域靶位近红外Ca2+荧光纳米传感器的研究
  • 项目名称：信号肽介导亚细胞区域靶位近红外Ca2+荧光纳米传感器的研究
  • 项目类别：面上项目
  • 批准号：20975012
  • 申请代码：B0509
  • 项目来源：国家自然科学基金
  • 研究期限：2010-01-01-2012-12-31
  • 项目负责人：张小玲
  • 负责人职称：教授
  • 依托单位：北京理工大学
  • 批准年度：2009
中文摘要：

基于信号肽的靶向定位作用，设计合成近红外新型Ca2+荧光探针，信号肽修饰的纳米微胶囊包被，制备亚细胞区域定位、易负载细胞Ca2+荧光纳米传感器，并应用于细胞Ca2+信使作用研究。该研究不仅为亚细胞水平Ca2+的实时动态监测提供有效的手段，其信号肽介导的靶向定位方法，也为用于细胞内其它生物活性物质测定的小分子荧光探针的靶向标记开辟崭新的途径。同时，近红外检测和纳米微胶囊包被将减小细胞自发荧光干扰和小分子探针的光漂白及细胞毒副作用影响。

中文主题词： 信号肽；亚细胞靶位；钙荧光探针；纳米传感器；近红外
英文摘要：

Signal peptide；Subcellular Targeting；Fluorescent Ca2+ probe；Nanosensors；Near-infared

英文主题词： Signal peptide；Subcellular Targeting；Fluorescent Ca2+ probe；Nanosensors；Near-infared
结论摘要：

设计合成了针对细胞Ca2+及其他生物活性物质的系列新型小分子荧光探针及功能化纳米晶，研究了其发光及分析性能。在此基础上，基于信号肽的靶向定位作用，用信号肽修饰的纳米微胶囊包被Ca2+荧光探针，制备亚细胞区域定位、易负载细胞Ca2+荧光纳米传感器，并应用于细胞Ca2+信使作用研究。该研究不仅为亚细胞水平Ca2+的实时动态监测提供有效的手段，其信号肽介导的靶向定位方法，也为用于细胞内其它生物活性物质测定的小分子荧光探针的靶向标记开辟崭新的途径。同时，近红外检测和纳米微胶囊包被将减小细胞自发荧光干扰和小分子探针的光漂白及细胞毒副作用影响。

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  • 期刊论文
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期刊论文
One-pot Self-Assembly of Flower-like Cu2S Structures with Near-Infrared Photoluminescent Properties
C_(60)室温荧光发射现象研究
One-pot synthesis of PbSe hollow single-crystalline nanoboxes based on gas bubble assisted Ostwald r
On the fluorescence of C-60 at room temperature
The Determination Of Thiols Based Using A Probe That Utilizes Both An Absorption Red-Shift And Fluor
Surface-enhanced Raman scattering of C60 on co-modified substrate of Fe3O4 and Au nanoparticles
Synthesis, Characterization and Photoluminescence of CdS Hyperbranched Nanocrystals by a Simple Solu
Engineering a Subcellular Targetable, Red-Emitting and Ratiometric Fluorescent Probe for Ca2+ and It
A colorimetric and ratiometric fluorescent probe for quantitative detection of GSH at physiologicall
Size-controlled synthesis of Au nanoparticles and nanowires and their application as SERS substrates
CdSe quantum dots decorated by mercaptosuccinic acid as ?uorescence probe for Cu2+
A 4-hydroxynaphthalimide-derived ratiometric fluorescent chemodosimeter for imaging palladium in liv
Synthesis and Characterization of CdS Nanoparticles in the Presence of Oleic Acid as Solvent and Sta
A colorimetric and ratiometric fluorescent probe for quantification of bovine serum albumin
Low temperature fabrication of flexible carbon counter electrode on ITO-PEN for dye-sensitized solar
A Highly Selective Colorimetric and "Off-on-off" Fluorescent Probe for Fluoride Ions
CdS纳米棒的制备、表征及其形成机理
Fabrication of high performance Pt counter electrodes on conductive plastic substrate for flexible d
1-Hexadecylamine as both Reducing Agent and Stabilizer to Synthesize Au, Ag Nanoparticles and Their
Baocun Zhu, Xiaoling Zhang*, Hongyin Jia, Yamin
Synthesis of SERS Active Au Nanowires in Different Noncoordinating Solvents
A highly selective colorimetric and ratiometric fluorescent chemodosimeter for imaging fluoride ion
Near-Infrared Light-Responsive Core-Shell Nanogels for Targeted Drug Delivery
A Highly Selective Wavelength-Ratiometric And Colorimetric Probe For Cysteine
Facile synthesis of Ni/Au‚ Ni/Ag hybrid magnetic nanoparticles New active substrates for surfa
Fluorescence detection of adenosine triphosphate through an aptamer–molecular beacon multiple
Oleylamine as solvent and stabilizer to synthesize shape-controlled ZnS nanocrystals with good optic
A highly selective colorimetric chemodosimeter for fast and quantitative detection of hydrogen sulfi
Facile synthesis of SERS active Ag nanoparticles in the presence of tri-n-octylphosphine sulfide
A simple but highly sensitive and selective colorimetric and fluorescent probe for Cu2+ ion aqueous
Synthesis of SERS active Ag2S nanocrystals using oleylamine as solvent‚ reducing agent and sta
Synthesis and Optical Properties of CdS nanorods and CdSe nanocrystals Using Oleylamine as both Solv
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张小玲的项目
可逆型近红外氧化还原荧光探针的合成及生物应用
近红外分子/纳米荧光探针制备及其在肿瘤分子成像分析中的应用研究