目的:对中药牛至有效部位挥发油中所含对-聚伞花素进行了含量测定,比较不同产地的含量差异,为该药材质量标准制定提供依据。方法:采用高效液相色谱法对牛至挥发油中对-聚伞花素的含量进行测定。结果:对-聚伞花素在4.388~21.940μg范围内,线性关系良好,平均回收率为97.50%,RSD为0.38%(n=5)。结论:该方法准确灵敏、稳定可靠。对-聚伞花素可作为控制牛至质量的一个指标成分。
OBJECTIVE To develop a method for the determination of p-Cymene in essence oil of Origannum Vulgate. METHODS An HPLC method was developed. RESULTS Linearity was found in the range of 4. 388~21. 940 g,the average recovery was 97. 50%, RSD was 0. 38% (n = 5). CONCLUSION This method is reliable, accurate and suitable for the determination of p-Cymene.