中文摘要：

目的：探讨细胞外基质蛋白1（extracellular matrix protein1,ECM1）在乳腺癌组织和细胞株中的表达及其意义。方法：应用免疫组织化学和免疫细胞化学EnVision法检测乳腺癌手术切除标本中乳腺组织（正常组织和癌组织）和恶性程度不同的乳腺癌细胞株（MCF-7、Sk-Br-3和MDA-435）中ECM1的表达,并比较正常乳腺组织和乳腺癌组织、淋巴结转移和未转移乳腺癌之间的ECM1表达差异。Western blotting检测确定ECM1亚型蛋白。结果：ECM1的表达在正常乳腺组织中阳性率为8.7%（2/23）,在乳腺癌组织中阳性率为60.4%（29/48）;其中乳腺癌淋巴结未转移者为38.5%（10/26）,淋巴结转移者为86.4%（19/22）。统计分析表明,ECM1的表达在乳腺癌组织中高于正常组织（P〈0.01）,在转移性乳腺癌中高于非转移性乳腺癌（P〈0.01）。恶性程度低的乳腺癌细胞株MCF-7、Sk-Br-3中不表达ECM1,而恶性程度较高的MDA-435细胞株中高表达ECM1。乳腺癌细胞中高表达的ECM1蛋白主要是相对分子质量为68000的ECM1a亚型。结论：ECM1（主要为ECM1a亚型）在乳腺癌组织和恶性程度高的乳腺癌细胞中过表达,并且与乳腺癌的淋巴结转移相关。

英文摘要：

Objective： To investigate the expression of extracellular matrix protein 1 （ECM1） in breast cancer tissues and cell lines and to assess its significance. Methods： Immunohistochemical and immunocytochemical EnVision methods were used to detect expression of ECM1 in the breast tissues（normal tissues and cancer tissues） and in breast cancer cell lines of different malignancies （ MCF-7, Sk-Br-3, and MDA-435）. The ECM1 positive rates were compared between normal tissues and cancer tissues and between cancer tissues with and without lymph node metastasis. Western blotting was used to identify the subtypes of ECM1. Results： The positive rate of ECM1 was 8.7% （2/23） in the normal breast tissues, 60.4% （29/48） in breast cancer tissues, 38.5% （10/26） in breast cancer tissues without lymph node metastasis, and 86.4% （19/22） in those with lymph node metastasis. Statistical analysis indicated that the ECM1 expression was higher in the breast cancer tissues than in the normal tissues （P 〈 0.01 ）, and the expression in the metastatic breast cancer was higher than that in the primary cancer tissues （P 〈0. O1 ）. Cell lines with low malignancy, such as MCF-7 and Sk-Br-3 cells, were negative of ECM1 ; cell lines with high malignancy, such as MDA-435 cells, were strongly positive of ECM1. Western blotting showed that the highly expressed ECM1 protein was mainly ECMIa subtype, with a relative molecular mass of 68 000. Conclusion： ECM1, mainly ECMla subtype, is over-expressed in breast cancer tissues and cell lines of high malignancy, which is associated with the metastasis of breast cancer.