中文摘要：

目的探讨Homer家族在注意缺陷多动障碍（ADHD）动物模型幼年自发性高血压（SHR）大鼠与Wistar京都（WKY）大鼠前额叶中的表达差异情况，分析其与ADHD的关系。方法2006年12月至2007年7月在南京医科大学儿科医学研究所应用反转录-聚合酶链反应（RT—PCR）、免疫蛋白印迹技术（Western Blotting）检测SHR与WKY大鼠前额叶皮质中Homer1a,Homet 1b／c、Homer2a／bmRNA和蛋白的表达情况。结果RT—PCR结果显示，SHR大鼠Homer 1a、Home2a/bmRNA表达量较WKY大鼠降低，差异有统计学意义（t=2．56，P〈0．05；t=5．85，P〈0．05），Homer 1b／c在两组间差异无统计学意义／t=0．44，P〉0．05）。Western Blotting结果显示，SHR大鼠Homer1a、Homer2a/b蛋白表达量较WKY大鼠降低，差异有统计学意义（t=2．76，P〈0．05；t=3．40，P〈0．05）。结论SHR大鼠前额叶皮质Homer1a、Homer2a／b表达较WKY大鼠显著降低，提示Homer1a、Homer2a/b可能与ADHD密切相关。

英文摘要：

Objective To investigate the expression of Homer on Attention-Deficit Hyperactivity Disorder （ADHD）. Methods The total RNA and protein were extracted from prefrontal cortex （PFC） of SHR and WKY rats. Both RT-PCR technique and Western Blotting technique were used to detect the expression levels of different isoforms of Homer （Homer 1a, Homer 1b/c, Homer 2a/b） in PFC of SHR and WKY rats. Results （1） By RT-PCR detection, it was found that the expression levels of Homer la and Homer 2a/b gene were significantly lower in prefrontal cortex of SHR rats than those in WKY ones （t = 2.56,P 〈 0.05;t = 5.85,P 〈 0.05）. There was no difference in the expression levels of Homer 1b/c gene in prefrontal cortex between SHR and WKY rats （t = 0.44, P 〉 0.05）. （2） By Western Blotting, it was found that the expression levels of Homer la and Homer 2a/b gene were significantly lower in prefrontal cortex and hippocampus of SHR rats than those in WKY rats （ t = 2.76, P 〈 0.05 ; t = 3.40, P 〈 0.05）. Conclusion Homer la and Homer 2a/b may play an important role in pathogenesis of ADHD.