中文摘要：

目的：探讨血管特异结合短肽CGNSNPKSC在胃癌中的表达及与肿瘤分化程度的关系;初步筛选短肽CGNSNPKSC的受体.方法：采用免疫组化SP法检测CGNSNPKSC在胃癌中的表达及与分化程度的关系;以CGNSNPKSC作为＂探针＂免疫筛选表达型λZAP噬菌体cDNA文库,筛选CGNSNPKSC可能的受体,并利用生物信息学对阳性克隆进行初步分析.结果：CGNSNPKSC受体在高,中,低度分化胃癌的血管中表达率依次为12/18（67%）,17/23（74%）,22/24（92%）;在低分化胃癌组织中的表达明显高于高分化和中分化胃癌组织（P＜0.05）.cDNA文库筛选获得43个独立候选克隆,生物信息学显示分属于13种不同的基因.结论：CGNSNPKSC与胃癌的血管特异结合,可作为肿瘤血管抑制治疗的靶向载体.

英文摘要：

Objective： To detect the expression of vascular - binding peptide CGNSNPKSC in gastric cancer and to identify its possible receptor. Methods： Immunohistochemical analysis was used to detect the expression of CGN- SNPKSC in gastric cancer and its relation with tumor type. A micro - vascular cDNA library was screened to detect the receptor of CGNSNPKSC, and the possible inserts were further identified by bioinformative assay. Results： CGNSNPKSC was found in 51/65 （78%） cases of gastric cancer tissues, including 12/18 （67%） cases of well- differentiated tissues, 17/23 （74%） cases of moderate - differentiated tissues and 22/24 （92%） cases of poor - differentiated tissues. Statistical analysis showed that there was significant correlation between the expression of CGNSNPKSC and the differentiation of gastric cancer. After four rounds of selection, 43 clones （designated C1 -CA3） were found showed reactivity to CGNSNPKSC. Sequence analysis and on - line homology analysis revealed that these inserts belonged to 13 genes. Conclusion： These results took further insight into the characteristic of CGNSNPKSC, and strongly suggested that CGNSNPKSC might be used as vehicle delivering cytokine or chemotherapy in targeting gastric cancer therapy.