Temporal and spatial profiling of nuclei-associated proteins upon TNF-α/NF-kB signaling
- ISSN号:1001-0602
- 期刊名称:《细胞研究:英文版》
- 时间:0
- 分类:Q78[生物学—分子生物学] Q257[生物学—细胞生物学]
- 作者机构:[1]Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
- 相关基金:Abbreviations: TNF (tumor necrosis factor); SILAC (stable isotope label- ing by amino acids in cell culture); LC-MS/MS (liquid chromatography tandem mass spectrometry); LTQ-Orbitrap (linear ion trap/Orbitrap); HPRD (Human Protein Reference Database); SMARC (SWI/SNF-related matrix-associated actin-dependent regulator
chromatin); KEGG (Kyoto Encyclopedia of Genes and Genomes)Acknowledgment We would like to thank Dr/an Scott (Department of Molecular Genetics, University of Toronto), Dr Chengjian Tu (Vanderbilt Medical Center, Vanderbilt University) and Ya-Wen Tian (Key Laboratory of Systems Biology, Shanghai Institutes for Biologi- cal Sciences) for critical reading of the manuscript. This work was supported by the National Natural Science Foundation of China (30425021, 30521005), Basic Research Foundation (2006CB910700), CAS Project (KSCX2-YW-R-106, KSCX1- YW-02) and High-technology Project (2007AA02Z334).
关键词:
肿瘤坏死因子, 信号通路, 相关蛋白, 监测时间, 空间特征, WESTERN印迹, HEK293细胞, 蛋白质组学, quantitative analysis, SILAC, proteomics, TNF-α/NF-kB, nucleus, FANCD2, subcellular fractionation
中文摘要:
Correspondence: Rong Zeng E-mail: zr@sibs.ac.cn