中文摘要：

以栽培草莓品种‘全明星’为试材，通过3，-和5LRACE技术克隆出miR390靶基因TAS3的cDNA全长，命名为FaTAS3。序列分析发现：草莓TAS3基因的cDNA全长为742bp，含有16个碱基的PolyA尾巴及2个高度保守的ta—siRNA产生位点和1个miR390靶位点；该基因DNA全长为824bp，5’端130bp处有一个98bp的内含子序列。生物信息学软件预测显示，草莓TAS3基因的启动子除具有TATA／CAAT—box外，还含有Gbox、C—box等特异作用元件。实时定量RT—PCR结果表明，草莓miR390与靶基因TAS3间的表达模式与拟南芥中的表达模式相同，推测草莓TAS3基因的生物合成也受miR390的指导。

英文摘要：

The miR390-targeted TAS3 gene named FaTAS3 was isolated from strawberry （Fragaria ananassa ＇Allstar＇） with the methods of PCR and RACE. Sequence analysis showed that the cDNA was consisted of a 742 base pair with a 16 bp poly A and highly conserved domains,two ta-siRNA-generating regions and one miR390 complementary site. The full-length DNA of FaTAS3 is 824 bp with an intron sequence of 98 bp at the t30 bp from the 5＇ end of TAS3 transcript. In addition to the TATA/CAAT-box,its promoter predicted by bioinformatic analyses also contained some specific regulatory elements such as G-box,C-box and etc. The results of real time RT-PCR showed that the expression pattern of miR390 and its targeted TAS3 gene in strawberry is a similar manner to Arabidopsis. So we deduce that the biological formation of strawberry TAS3 gene was also cleaved by miR390.