中文摘要：

目的：研究肿瘤坏死因子I型受体（tumor necrosis factor receptor typeI，TNFR—I）在大鼠颈动脉体（ca-rotid body，CB）中的表达，以及TNF-α对CB球细胞胞内钙离子浓度（Ca2＋，）的影响。方法：Western Blot和免疫荧光双重染色检测TNFR—I在大鼠CB中的表达，用钙成像技术检测TNF-α对大鼠CB球细胞Ca2＋；的影响。结果：WesternBlot结果显示，TNFR—I阳性条带出现在55kD处，与其分子量一致。免疫荧光双重染色结果显示，TNFR—I强烈表达在大鼠CB球细胞中。钙成像结果显示，外源性给予TNF-α可引起球细胞Ca2＋i迅速升高。结论：大鼠CB球细胞表达TNFR—I，可以感受促炎性细胞因子TNF-α的刺激。

英文摘要：

Objective： To study the expression of tumor necrosis factor receptor type I （TNFR-I） in the rat carotid body （ CB）, and the effect of TNF-α on intraeellular Ca2 ＋ （ [ Ca2 ＋] i ） level in the glomus cells of the rat CB. Methods ： West- ern Blot and double immunofluorescent double staining methods were used to investigate the expression of TNFR-I in the rat CB. Calcium imaging was used to observe the effect of TNF-oL on [ Ca2 ＋]i level in the glomus cells of the rat CB. Re- sults： The result of Western Blot shwed that TNFR-I protein band appeared at 55 kD, consistent with the molecular weight of the receptor. The result of double immunofluorescent staining showed that TNFR-I was expressed in the CB glo- mus cells. The result of calcium imaging showed that extracellular application of TNF-α induced a rise in [ Ca2＋ ] i level in the cultured glomus cells of the rat CB. Conclusion： TNFR-I is strongly expressed in the glomus cells of the rat CB, and the glomus cells can sense the stimulation of proinflammatory cytokine TNF-α.