中文摘要：

目的建立同时测定泽泻中泽泻醇C、泽泻醇F、23-乙酰泽泻醇C、泽泻醇L、24-乙酰泽泻醇F、泽泻醇A、24-乙酰泽泻醇A、泽泻醇G、泽泻醇B、23-乙酰泽泻醇B和11-去氧泽泻醇B 11个三萜类成分的RP-HPLC-DAD分析方法。方法采用Ultimate XB-C18色谱柱（150 mm×4.6 mm,5μm）,流动相乙腈（A）-水（B）进行梯度洗脱,体积流量1 m L/min,检测波长为208、245 nm,柱温30℃。比较25批泽泻中11个三萜类成分的量。结果泽泻醇C、泽泻醇F、23-乙酰泽泻醇C、泽泻醇L、24-乙酰泽泻醇F、泽泻醇A、24-乙酰泽泻醇A、泽泻醇G、泽泻醇B、23-乙酰泽泻醇B、11-去氧泽泻醇B 11个成分的线性范围分别为0.313-17.210μg/m L（r=0.999 9）、0.504-11.880μg/m L（r=0.999 9）、0.284-9.369μg/m L（r=0.999 9）、0.146-2.680μg/m L（r=0.999 9）、0.254-5.596μg/m L（r=0.999 8）、2.500-66.000μg/m L（r=0.999 8）、0.463-10.190μg/m L（r=0.999 9）、1.575-20.475μg/m L（r=0.999 9）、1.525-57.268μg/m L（r=0.999 6）、3.035-118.362μg/m L（r=0.999 9）、0.816-16.880μg/m L（r=0.999 8）。平均加样回收率分别为98.76%、100.24%、97.97%、100.14%、99.88%、96.54%、97.27%、98.85%、99.45%、98.85%和98.13%。结论该法准确、可靠、分离效果良好,为泽泻药材质量评价提供了可靠的方法。

英文摘要：

Objective To establish a RP-HPLC-DAD method for simultaneous determination of alisol C, alisol F, alisol C 23-acetate, alisol L, alisol F 24-acetate, alisol A, alisol A 24-acetate, alisol G, alisol B, alisol B 23-acetate, and 11-deoxy-alisol B in Alismatis Rhizoma. The content of 11 components from 25 batches of samples collected from different habitats was determined and compared by means of this established method. Methods The separation was achieved on a Ultimate XB-C18 column （150 mm × 4.6 mm, 5 μm） with the mobile phase consisting of water-acetonitrile to be gradient elution. Flow rate was 1 mL/min and column temperature was 30 ℃. The optimum detection wavelengths were 208 and 245 nm. Results The linear ranges of alisol C, alisol F, alisol C 23-acetate, alisol L, alisol F 24-acetate, alisol A, alisol A 24-acetate, alisol G; alisol B, alisol B 23-acetate, and ll-deoxy-alisol B were 0.313- 17.210 （r = 0.999 9）, 0.504-11.880 （r = 0.999 9）, 0.284-9.369 （r = 0.999 9）, 0.146-2.680 （r = 0.999 9）, 0.254-5.596 （r = 0.999 8）, 2.500-66.000 （r = 0.999 8）, 1.620-35.640 （r = 0.999 9）, 1.575-20.475 （r = 0.999 9）, 1.525-57.268 （r = 0.999 6）, 3.035-118.362 （r = 0.999 9）, and 0.816-16.880 μg/mL （r = 0.999 8）, respectively. The average recoveries （n = 6） were 98.76%, 100.24%, 97.97%,100.14%, 99.88%, 96.54%, 97.27%, 98.85%, 99.45%, 98.85%, and 98.13% respectively. Conclusion The RP-HPLC-DAD method is feasible and accurate, which could provides the scientific evidence for quality control ofAlismatis Rhizoma.