中文摘要：

以泽泻提取物中总三萜（1）和23-乙酰泽泻醇B（2）含量为评价指标;优化泽泻前处理、提取和纯化方法.所得最优工艺为：取过10目筛的泽泻粉末,用10倍量85％乙醇回流提取2次,每次1h.所得提取液浓缩至每1 ml含0.5 g生药的溶液,取100 ml以2 BV/h （60 ml/h）的速度上D101大孔吸附树脂柱,上样完成后用水4 BV洗脱,再用6 BV 40％乙醇以4 BV/h的速度除杂,8BV 80％乙醇以2BV/h的流速富集1.实验室小试至50kg规模的中试试验表明,该工艺稳定可行,所得提取物中1含量大于50％,得率约77％,2的含量约为8％.

英文摘要：

The pretreatment, extraction and purification methods of Alisma orientalis （Sam.） Juzep. were optimized with contents of total triterpenes （1） and alisol B 23-acetate （2） as indexes. The optimized process was as follows： the crude herbs about 10 mesh were extracted under reflux by 10 times of 85 % ethanol twice, 1 h pre time. The extract containing crude drug 0.5 g/ml with the volume of 100 ml was loaded on the D101 macroporous resin column with the sample flow rate of 2 BV/h （60 ml/h）. Then the column was washed by 4 BV of water, 6 BV of 40 % ethanol with the rate of 4 BV/h for removing impurities and 8 BV of 80 % ethanol with 2 BV/h for purifying 1. Both the laboratory scale test and pilot scale test of 50 kg showed that the optimal process was stable. Contents of 1 and 2 in the extract obtained by this process were above 50 % and 8 %, the yield of 1 was about 77 %.