中文摘要：

以纽荷尔脐橙和山下红温州蜜柑为材料,研究不同水分胁迫处理对柑橘内源茉莉酸合成及相关酶基因表达的影响。采用提取总RNA与RT-PCR方法合成cDNA,通过荧光定量PCR检测茉莉酸合成途径中相关酶基因（CitLOX、CitAOS、CitAOC）在不同水分胁迫处理下的表达模式。试验结果表明,经过水分胁迫处理后柑橘叶片的内源茉莉酸、脯氨酸、可溶性糖含量随着水分胁迫程度的增加逐渐升高,但是叶绿素随着胁迫程度增加而逐渐降低。CitLOX与CitAOC基因在轻度水分胁迫的条件下,基因表达量较高,随着水分胁迫程度增加表达量降低;CitAOS基因随着水分胁迫程度的增加其表达量逐渐增加,在重度水分胁迫条件时,基因的表达量最高,并与JA含量呈现显著正相关,CitAOS基因在柑橘茉莉酸合成途径中可能发挥着更为重要的作用。

英文摘要：

The experiment used Yamasitaka[Citus.unshin Marc]and Newhall navel orange[Citus.sinensis（L） Osbeck]as materials to study the role of correlative enzymes and correlative gene expression in endogenous JA biosynthesis under water stress.The total RNA obtained from leaf of Citrus was successfully used to amplify cDNA sequences and RT-PCR,and q-PCR methods were used to detect and analyze correlative enzyme genes（such as CitLOX,CitAOS and CitAOC） expression.The results showed that the accumulation of JA,content of proline and soluble sugar were significantly increased by water stress,however,content of chlorophyll decreased with the increasing of water stress.Real-time quantitative PCR was used to analyze the correlative genes＇expression in endogenous JA biosynthesis pathway,the result showed that CitLOX and CitAOC expression was high under slight water stress treatments,but it was low under severe water stress treatments.CitAOS expression increased gradually with water stress,there was significantly positive relationship between content of JA and CitAOS expression,it indicated that CitAOS may play more important role than those genes involved in JA biosynthesis in citrus.