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中文摘要：
目的 探讨线粒体呼吸链三磷酸腺苷合成酶6、8（ATPase6、8）与早产新生大鼠高氧肺损伤的关系。方法 早产新生SD大鼠生后1d随机分为空气组、高氧组。高氧组持续暴露于常压氧舱中，氧体积分数〉850mL／L；空气组置于同一室常压空气中。二组分别于实验后1、4、7、10和14d提取其肺组织RNA．采用半定量逆转录聚合酶链反应（RT-PCR）测定ATPase6、8mRNA表达。结果 1．与空气组比较，高氧暴露1dATPase6mRNA表达显著增强（P〈0．05）；4、7、10d时无显著性差异（Pa〉0．05）；14d又显著增强（P〈0.01）。2．高氧组与空气组比较，ATPase8mRNA表达于1、4、10d时无显著改变（Pa〉0．05）；7、14d时明显增强（Pa〈0．05）。结论 高体积分数氧暴露诱导线粒体呼吸链中ATPase6、8异常表达，这种变化可能参与早产新生大鼠高氧肺损伤的发病过程。
英文摘要：
Objective To investigate the relationship betweent ATPase6,8 and hyperoxia -induced lung injury in premature newborn rats. Methods The 1 - day - old preterm Sprague - Dawley （SD） rats were randomly assigned into air group and hypemxia group. Hyperoxia group were continuously exposed to oxygen （ oxygen 〉 850 mL/L） and air group in room air. After 1,4,7,10,14 days of exposure, the rats were killed, whole lung RNA were isolated and ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction（ RT - PCR）. Results 1. Compared with air group, ATPase6 mRNA expression was significantly increased （ P 〈0. 05 ） after 1 day of exposure ; it did not significantly change at day 4,7,10（ Pa 〉0.05 ） ;on the 14^th day ATPase6 mRNA expression significantly increased（ P 〈 0.01 ）. 2. Compared with air group, ATPase8 mRNA expression did not significantly change（ Pa 〉 0.05 ）after 1,4,10 days of exposure;on the 7^th, 14^th days, ATPase8 mRNA expression significantly increased（Pa 〈0.05）. Conclusion Hyperoxia induces changes of construction and function of ATPase6,8, which is likely to participate in the pathogenesis of hyperoxia - induced lung injury.
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