中文摘要：

目的研究高级氧化蛋白产物（AOPPs）为何种蛋白氧化产物并进行表征。方法羰基用于评定AOPPs中的氧化蛋白。天然血浆测得其蛋白浓度后，先按定义测定其AOPPs水平。血浆随即经超滤膜超滤、PBS清洗后制得血浆-AOPPs。排阻色谱法用于分析定量单个蛋白中的羰基水平。另用氯仿对血浆进行脱脂。脱脂产生的馏分同样进行羰基水平等的测定。结果无论是天然血浆、脱脂血浆或是氯仿脱脂产生的沉淀中，尿毒症患者的AOPPs和总羰基水平始终明显高于正常人。HPLC分析证明血浆白蛋白不仅具有最高的羰基水平，而且也是唯一一种在正常人和患者间差异具有统计学意义的蛋白。脱脂主要去除的是纤维原蛋白和一种未明大分子蛋白，这2种蛋白的氧化程度均较低。结论白蛋白是AOPPs的主要构成成分。而其它蛋白占AOPPs的构成比例较小，特别是IgG。

英文摘要：

Objective To design a method to characterize AOPPs. Methods Carbonyl groups were used as an oxidative index to hnk AOPPs and oxidized protein. Plasma-AOPPs were obtained by a series of preparation as follows. The native plasma was first determined for its protein contents followed by AOPPs level evaluation. Specimen was then washed with PBS and uhrafiltratod with an uhrafilter （10 000 cut-off membrane） to obtain clean ptasma-AOPPs. A size-exclusion HPLC technique was used to verify which protein was oxidatively damaged. Fractions resulted from dehpidation were also examined. Results The levels of AOPPs and total carbonyl groups in patient plasma were significantly higher than those in controls; both in native/dehpidated plasma and CHCl3-resulted precipitate. HPLC revealed that serum albumin presented highest carbonyl levels. It was an exclusive protein with statistically significant difference between controls and patients （patients vs. controls in nmol carbonyl/mg protein： HSA： 1.510±0.067 vs. 0.791±0.048, P 〈 0.01 ; Fg： 0.617±0.100 vs. 0.672±0.159, P= 0.773; IgG： 0.047±0.029 vs. 0.053±0.030, P= 0.791; Tf： 0.102±0.025 vs. 0.098±0.043, P = 0.385, respectively）. Dehpidation mainly removed fibrinogen and an unknown oxidized macromolecule. Conclusion Albumin is a more important contributor to AOPPs than other proteins, IgG in oarticular.