中文摘要：

目的探讨自由基攻击对白蛋白结构的影响。方法以正常人为对照组，尿毒症患者为实验组，高级氧化蛋白产物（AOPPs）和羰基为氧化应激指标。先对各组的氧化应激水平进行评价，随后从各组分离、纯化出白蛋白，再对其氨基酸组成进行分析比较。结果尿毒症患者的AOPPs，及羰基水平约为正常人的2倍（P〈0.01）。同正常人相比，尿毒症患者水解出的17种白蛋白氨基酸组成大部分低10％左右，其中胱氨酸和精氨酸在两组间差异有统计学意义（P〈0．05）。结论氨基酸的普遍损失是影响血浆蛋白结构和功能的关键因素，其中胱氨酸和精氨酸是自由基攻击的两个主要靶基团。

英文摘要：

Objective To study the effects of oxidants on the structure of albumin. Methods Using both AOPPs and protein carbonyl content as indices. The oxidative stress level in normal controls and uremia patients was evaluated. Albumin in plasma was purified by HPLC and then was subjected to amino acids composition assay. Results Both AOPPs level and protein carbonyl content in uremic patients were significantly higher than those in controls （P 〈 0.01 ）. Most amino acids＇ levels in albumin from uremic patients were down-regulated by 10% compared to the controls. A statistically significant difference of both cysteine and arginine in albumin was found between controls and uremic patients （P 〈 0.05）. Conclusion The extensive loss of amino acids in uremic patients may play a pivotal role in affecting the structure and biological functions of albumin, provided that both cysteine and arginine are the major targets attacked by free radicals.