中文摘要：

利用RT-PCR技术从山荆子cDNA中克隆CBF基因,ORF全长756 bp,编码252个氨基酸;其氨基酸序列含典型的CBF基因蛋白保守的序列AP2/EREB DNA结合域及CBF家族蛋白特征短多肽序列（PKK/RPAGRxKFxETRHP和DSAWR）;与Genebank上收录的几种植物CBF基因进行氨基酸同源性比对,结果发现山荆子CBF转录因子MbCBF和苹果CBF/DREB1基因的氨基酸序列相似性为98%;与梅树CBF相似性为67%;进一步构建了植物表达载体,为利用CBF基因提高植物抗逆性奠定基础。

英文摘要：

The CBF transcription factors were cloned by RT-PCR technique from the plants of Malus baccata.The full-length of open reading frame （ORF） was 756 bp, encoding 252 amino acids;The amino acids sequence owned the characteristics of CBF protein,which contained an AP2/EREB DNA binding domain and two special short amino acids sequences ;The amino acids sequences of MbCBF had highly homologous with CBF genes of other plants included in Genebank ,reached 98% with Malus domestica,and 67% with Prunus mume respectively;Then we connected the gene into the plant expression vector PB1121,which laid the foundation for the utilization of CBF genes to improve plant stress tolerance.