中文摘要：

目的建立RP-HPLC测定忍冬藤中绿原酸、咖啡酸、当药苷和马钱子苷含量的方法，并以此对忍冬藤进行质量控制。方法色谱柱为Aglient Extend，C18（4．6mm×250mm，5μm）；流动相为甲醇-0．4％醋酸水溶液（26：74）；流速1．0mL·min^-1，检测波长240nm。结果绿原酸、咖啡酸、当药苷和马钱子苷的线性范围和相关系数分别为23．5～470mg．L^-1，r=0．9999；12～240mg·L^-1，r=0．9997;10．5～210mg·L^-1，r=0．9999；13-260mg·L^-1，r=0．9998。平均回收率分别为97．7％（RSD=2．23％），96．6％（2．29％），95.0％（2．26％），103.3％（2．93％）。结论本方法简单、快速、可靠，可用于忍冬藤药材的质量控制。

英文摘要：

OBJECTIVE To establish a RP-HPLC method for simultaneous determination of four bioactive compounds in Caulis Lonicerae Japonicae, i.e. chlorogenic acid, caffeic acid, sweroside and loganin. METHODS The separation was performed on an Aglient Extend-C18 column （4.6 mm×250 mm, 5μm）, using a isocratic elution with methanol-water （containing 0.4% acetic acid） as the mobile phase. The flow rate was 1.0 mL·min^-1, the detection wavelength was set at 240 nm. RESULTS The linear ranges and regression equations of chlorogenic acid, caffeic acid, sweroside and loganin were 23.5-470 mg·L^-1 with r=0.999 9; 12-240 mg·L^-1 with r=0.999 7; 10.5-210 mg·L^-1 with r=0.999 9 and 13-260 mg·L^-1 with r=0.999 8, respectively. The average recoveries （n=6） ofthe four constituents were 97.7% （RSD=2.23%）, 96.6% （2.29%）, 95.0% （2.26%）, 103.3% （2.93%）, respectively. CONCLUSION This method is simple, rapid and reliable for the quality control of Caulis Lonicerae Japonicae.