利用爱字棉1517×德州047重组近交系(recombinant inbred line,RIL)中G6群体构建的SSR遗传连锁图谱及基于混合线性模型的复合区间作图法对QTL进行定位,并对主效QTL,加性×加性上位性QTL及与环境互作效应进行分析,为利用分子聚合方法提高产量提供理论依据。对2006年、2008年以及2009年的产量性状进行分离分析,检测到24个不同年份的主效QTL,其中相关于单株籽棉、单株皮棉、衣分、子指以及单株铃数的分别检测到1个不同年份稳定存在的主效QTL;对3年的产量性状作环境因子联合分析,检测到14个主效QTL,其中6个与环境互作,检测到20对加加上位性QTL,其中7对与环境互作。不同年份检测的稳定且受环境影响小或不受环境影响的与近处标记紧密连锁的主效QTL可用于分子标记辅助选择,以提高育种的效率。
The genetic linkage map of SSR,constructed by mixed linear model composite interval mapping with CRI-G6(Acala 1517×Dezhou 047)population was used to detect and localize QTLs,including main-effect QTLs,epistasis QTLs and Q×E interaction effects in order to provide information applicable to cotton MAS(molecular marker assisted selection breeding).In a separate analysis,24 major QTLs for yield traits were identified in the three different years.Each stable major QTL was detected for seed yield,lint yield,lint percentage,seed index and bolls per plant,respectively.Fourteen main-effect QTLs and 20 pairs of additive-additive epistasis QTLs were detected by joint analysis in three years,among them six main-effect QTLs and seven pairs of additive-additive epistasis QTLs interacted with environments.These stable main-effect QTLs with a large effect(accounting for over 10%of phenotypic variation),which were scanned in different years and linked closely with markers,can be used for MAS in high-yield breeding program.