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陆海BC4F2和BC4F3代换系的评价及纤维产量与品质相关QTL的检测
  • ISSN号:0578-1752
  • 期刊名称:《中国农业科学》
  • 时间:0
  • 分类:S511[农业科学—作物学]
  • 作者机构:[1]中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南安阳455000
  • 相关基金:基金项目:国家“973”计划项目(2010CB126000)、中央级公益性科研院所基本科研业务专项(SJA0909)
中文摘要:

【目的】利用SSR标记对陆海BC4F2和BC4F3代换系进行评价并检测纤维产量与品质相关的QTL,为筛选棉花染色体单片段代换系、精细定位纤维品质QTL、实现分子聚合育种奠定基础。【方法】利用GGT32(graphical genotyping)软件分析每个代换系的基因型组成,采用SASPROCGLM的单向方差分析方法检测影响各性状的QTL。【结果】检测到50个单片段代换系,其中9株含有纯合的海岛棉片段,并筛选出12个代换片段少、纤维品质优良的代换系。共检测到15个控制产量性状和19个控制纤维品质的QTL,集中分布在12个连锁群中,解释的表型变异率在2.80%-14.13%。[结论]4个上半部平均长度QTL在2个世代中稳定遗传,1个上半部平均长度QTL在前人研究论文中检测到,部分标记位点同时控制几个不同的性状,并发现增效基因不全来自高值亲本。

英文摘要:

[Objective] A total of 276 SSR makers were used to assess the substitution lines and identify the QTLs related to fiber yield and quality traits in BC4F2 and BCaF3 populations from Gossypium hirsutum xGossypium barbadense with Gossypium hirsutum as recurrent parent, which could lay a foundation for further screening SSSLs (single segment substitution lines), finely mapping QTL of fiber yield and quality-related traits and realizing molecular assistant breeding. [Method] GGT32 was employed to analyze the genotype of BC4F2 and BCaF3 individuals. Possible QTL were identified by one-way analysis of variance on SAS PROC GLM. [Result] Atotal of 50 SSSLs were detected in BC4F2 and BCaF3 individual populations, in which 9 plants contained the single homozygosis chromosome segment of Gossypium barbadense. Twelve plants were screened out with excellent fiber quality trait and a few chromosome segments from Gossypium barbadense. Fifteen QTLs for fiber yield-related traits and 19 QTLs for fiber quality-related traits were mapped in twelve linkage groups, which accounted for 2.80%-14.13% of phenotypic variance. [ Conclusion] This study indicated that 4 QTLs related to fiber length could be detected in the two populations and 1 QTL for fiber length was reported in previous research, pleiotropism or linked genes existed in some loci that controlled different traits, and not all of additive genes were from the parent with excellent traits.

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620