中文摘要：

根据Gen Bank中猪链球菌（Streptococcus suis,Ss）2、7、9血清型的荚膜多糖基因簇中的斜基因序列,分别设计3对引物,在完成最佳条件筛选、特异性、敏感性试验的基础上,建立了一种快速区分猪链球菌2、7、9三种血清型的多重PCR检测方法,并对我国广东省分离鉴定的猪链球菌进行分型鉴定。结果表明,建立的多重PCR检测方法分别对猪链球菌血清2型、7型和9型扩增出特异性片段,片段大小分别是360、600、800 bp。该方法特异性良好,与副猪嗜血杆菌（Haemophilus parasuis,Hps）、多杀性巴氏杆菌（Pasteurellamultocida,Pm）、波氏杆菌（Brodetella bronchiseptica,Bb）、沙门氏菌（Salmonella,Sal）、大肠杆菌（Escherichia coli,E.coli）以及猪链球菌其他29个血清型均无交叉反应。用建立的多重PCR方法对临床分离的341株猪链球菌菌株进行血清型分型鉴定,并与传统的血清凝集分型结果进行比较,二者符合率为97.36%。与传统的血清凝集方法相比,本研究建立的PCR方法具有快速、灵敏、特异等特点,可应用于实验室的快速诊断以及猪链球菌的流行病学调查。

英文摘要：

Three pairs of primers were designed for development of a multiple PCR assay according to the sequences of capsular polysaccharide synthesis loci of Streptococcus suis serotypes 2, 7 and 9 published in GenBank. The clinical isolates of Streptococcus suis obtained from Guangdong province were used to test the specificity, sensibility and stability of the multiple PCR. The results showed this multiple PCR identified serotypes 2, 7 and 9. The specific PCR fragments for serotypes 2, 7 and 9 were 360, 600 bp and 800 bp, respectively. Specificity of the multiple PCR was determined using the clinical isolates of Haemophilus parasuis, Pasteurella multocida, Brodetella bronchiseptica, Salmonella, Escherichia coil and reference strains of other 29 serotypes of Streptococcus suis. Total 341 clinical isolates of serotypes 2, 7 and 9 of Streptococcus suis were tested using the multiple PCR and traditional serum agglutination and the agreement of these two methods was 97.36%. As compared with the traditional method, the multiplex PCR assay developed in this study was a rapid, sensitive and specific method, therefore it was suitable for the diseases surveillance and epidemiologieal diagnosis of Streptococcus suis.