为探明2014年广西猪群主要疫病的感染情况,本研究从发病猪场和屠宰场采集猪组织样品共325份,应用RT-PCR方法检测猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪流行性腹泻病毒(PEDV),并应用PCR方法检测猪圆环病毒2型(PCV2)和猪伪狂犬病病毒(PRV)。结果发现,发病猪场中,这5种病毒感染率分别为12.00%、28.57%、19.43%、53.71%和9.71%,而屠宰场的感染率分别为5.33%、2.67%、5.33%、59.33%和11.33%。对猪群混合感染情况分析发现,PCV2和其他病原的混合感染率最高。其中,发病猪场二重感染最高的为PRRSV+PCV2,达到11.43%,其次为PEDV+PCV2、CSFV+PCV2和PCV2+PRV,阳性率分别为5.71%、4.00%和4.00%;三重感染率最高的为PRRSV+PEDV+PCV2以及PRRSV+PCV2+PRV,阳性率均为2.29%。屠宰场二重感染最高的是PCV2+PRV,达到3.33%;三重感染最高的是CSFV+PCV2+PRV,阳性率为0.67%。结果表明,在发病猪场和屠宰场中,PCV2的感染率最高,且常与其他病原发生混合感染,PRV感染率呈上升趋势,加强对这2种病毒的监控对控制广西地区猪群发病具有重要意义。
To understand the current status of common swine diseases in Guangxi,total 325 pig tissue samples were collected from morbidity farms and slaughter houses.CSFV,PRRSV and PEDV were detected by RT-PCR,PCV2 and PRV were detected by PCR.The results showed that the infection rates of those viruses in morbidity farms were 12.00%,28.57%,19.43%,53.71%and 9.71%,respectively,and in the slaughter houses were 5.33%,2.67%,5.33%,59.33% and11.33%,respectively.The rate of PCV2 coinfection with other virus was the highest.In the morbidity farms,PRRSV+PCV2was the most universal double infection,which was 11.43%,posteriorly was PEDV+PCV2,CSFV+PCV2and PCV2+PRV,which were 5.71%,4.00% and4.00%,respectively,and PRRSV+PEDV+PCV2and PRRSV+PCV2+PRV were the most universal tripartite infection,both of them were 2.29%.In the slaughter houses,PCV2+PRV was the most universal double infection,which was 3.33%,CSFV+PCV2+PRV was the most uni-versal triple infection,which was 0.67%.These results indicated that PCV2 had the highest infection rate not only in morbidity farms but also in slaughter houses.PCV2 coinfection with other virus was universal.The infection rate of PRV has risen.It was important to enhance the surveillance of PCV2 and PRV for the control of swine disease in Guangxi.