中文摘要：

目的探讨胃癌中人端粒保护蛋白1基因（hPOT1）IVS13-98G／T位点单核苷酸多态性与幽门螺杆菌（H．pylori）感染的关系。方法采用聚合酶链反应和限制性片段长度多态性技术（PCR—RFLP）对150例外科手术切除的胃癌患者和156例正常对照者行基因型分析，采用PCR和Warthin-Starry银染色法检测幽门螺杆菌。结果胃癌组hPOT1 IVS13—98T／G位点GG、GT、TT基因型的频率分别22．00％、41．67％、36．67％，G、T等位基因频率分别为42．67％、57．33％；对照组GG、GT、TT基因型的频率分别为24．36％、51．92％、23．72％，G、T等位基因频率分别为49．68％和50．32％。两组G、T等位基因型频率比较无统计学意义（Х^2=3．0257，P=0．0820）。以T／T基因型作为参照基因型，G／T和G／G两种基因型OR值分别为0．432（95％CI：0．242—0．772，P=0．005）、0．540（95％CI：0．274—1．064，P=0．075）。胃癌中3种基因型H．pylori的检出率无统计学意义（P〉0．05）。结论hPOT1 IVS13—98G／T单核苷酸多态性可能与甘肃胃癌高发区的胃癌患者有关，可能为胃癌的保护因素。胃癌hPOT1 IVS13—98G／T单核苷酸多态性与H．pylori感染无关。

英文摘要：

Objective To explore the relationship between the single nucleotide polymorphisms （SNPs） of IVS13-98G/T of human protection of telomeres 1 （hPOT1） genes and H. pylori infection. Methods A total of 150 cases of gastric carcinoma and 156 cancer-free controls were genotyped by polymerase chain reaction （PCR） and PCR-restriction fragment length polymorphism （PCR-RFLP） method. H. pylori was identified with PCR and Warthin-Starry methods. Results The frequencies of GG, GT, and TT genotypes of hPOT1 IVS13-98 G/T were 22.00%, 41.67% and 36.67% in patients and 24.36%, 51.92%, and 23.72% in controls, respectively. The OR for any GT and GG genotype was 0.432（95% CI= 0.242-0.772, P=0.005） and 0.540 （95% CI= 0.274-1.064, P=0. 075） when compared with TT genotype, respectively. There were no differences in genotype distribution and allele frequency of IVS13-98 G/T polymorphism （Х^2 =3.0257, P= 0.0820）. hPOT1IVS13-98 G/T polymorphism was not correlated with H. pylori infection in gastric cancer. Conclusion Our results indicated that IVS13-98 G/T SNP of hPOT1 gene might be associated with reduced risk for gastric carcinoma, but not with H. pylori infection.