中文摘要：

目的研究表没食子儿茶素没食子酸酯（EGCG）对鼻咽癌细胞增殖和凋亡的影响及其分子机制。方法采用CCK-8法检测EGCG对鼻咽癌细胞株CNE-2Z增殖的影响;AnnexinV/PI双标记流式细胞术检测EGCG的凋亡诱导作用;实时荧光定量PCR和Western blot检测核转录因子E2F-1及其上游调控蛋白CyclinD1和p21的表达水平。结果 EGCG处理后,CNE-2Z细胞的增殖明显受到抑制,其细胞凋亡率亦显著增高,呈量效关系;随着EGCG浓度增高,E2F-1 mRNA和蛋白表达水平明显下降,其上游调控蛋白CyclinD1的表达亦明显下调,而p21的表达则明显上调。结论 EGCG可明显抑制鼻咽癌细胞增殖和诱导细胞凋亡,可能与其直接或间接下调核转录因子E2F-1有关,CyclinD1和p21参与其调控。

英文摘要：

Objective To study the effects of epigallocatechin-3-gallate（EGCG）on proliferation and apopto sis of nasopharyngeal carcinoma cell and its mechanism. Methods The growth and proliferation of CNE- 2Z cell was detected by CCK-8 assay,and apoptosis was assessed by AnnexinV/PI flow cytometry. The expressions of nuclear transcription factor E2F-1 and its regulatory protein CyclinD1 and p21 were detec ted by real-time quantitative PCR and Western blot. Results The proliferation of CNE-2Z cell was inhibited in a dose-dependent manner after treatment with EGCG,the apoptosis was induced as well. The expression of E2F-I mRNA and protein in CNE-2Z cell were markedly suppressed by EGCG, and the level of its regulatory protein CyclinDl also decreased while p21 was increased significantly. Conclusion EGCG suppressed the proliferation and induced apoptosis in CNE-2Z cell by directly or indirectly downregulating E2F 1 ,which was regulated by CyclinD1 and p21.