为研究氯酸盐抑制剂对SBR活性污泥中微生物的影响,采用序批式活性污泥反应器(SBR),在低温(15℃)条件下通过向反应器定时投加氯酸盐抑制剂,成功抑制系统中亚硝酸盐氧化菌(NOB)的活性,氨氮去除率和亚硝酸盐积累率均达到90%以上,实现了城市污水短程硝化的快速启动.采用实时荧光定量PCR对氨氧化菌(AOB)、亚硝酸盐氧化菌(NOB)和总细菌进行菌群定量分析,分析结果表明,氯酸盐抑制剂对NOB具有选择抑制性,对AOB的活性没有明显影响,而对NOB的活性抑制在短时间内是不可恢复的.
An activated sludge sequencing batch reactor(SBR) was developed to investigate the nitrogen remov- al from municipal wastewater based on the partial nitrification. SBR added chlorate was applied to inhibit the ac- tivity of nitrite-oxidizing bacteria (NOB) in low temperature. The results showed that the NH+4 - N removal effi- ciency and the nitrite accumulation rate of effluent were all above 90%. The quantitative PCR was used to ana- lyze the quantification of ammonia oxidizing bacteria( AOB), nitrite-oxidizing bacteria(NOB) and total bacteria in SBR. The results of real-time PCR revealed chlorate has its selective inhibition on NOB, without affecting the growth rate of AOB. It was also found that NOB inhibition caused by chlorate can't recover in a short time.