中文摘要：

目的：探讨β-NGF对骨缺损区新骨形成和改建的影响。方法：利用手术方法建立大鼠颅骨骨缺损模型,采用微渗透泵于骨缺损区局部持续导入外源性β-NGF。通过H-E染色、Gomori改良三色染色技术,检测术后3、7、14、21和28 d不同时间点骨缺损区的新生骨量,探讨β-NGF对骨缺损区新骨形成和改建的影响。应用Image-Pro Plus 6.0软件对骨缺损区中兴趣区域的新骨形成进行半定量测定,采用SPSS 19.0软件包对所得数据进行统计分析。结果：成功建立大鼠颅骨骨缺损局部持续导入β-NGF给药模型。H-E染色结果显示,导入β-NGF侧（实验组）与无β-NGF导入侧（对照组）均有新骨形成。Gomori改良三色染色结果发现,21、28 d时,实验组与对照组的新骨形成呈现明显增加,且实验组的新骨形成量显著多于对照组（P〈0.05）,28 d时的成熟骨量实验组显著多于对照组（P〈0.05）。结论：骨缺损区导入外源性β-NGF可能会促进新骨形成与骨改建。

英文摘要：

PURPOSE： Standard rat skull critical bone defects were made and locally continuous infusion of β-NGF animal model was conducted. The effect of β-NGF on bone formation and bone remodeling was evaluated. METHODS： Two 5 nun diameter bone defects were created on the parietal bone of SD rats, the fight side was given 10 μg β-NGF PBS solution（experimental group） and the left side was only given PBS solution（control group） via osmotic pump for 7 days. New bone formation was evaluated using H-E staining and Gomori modified trichrome staining. New bone formation in the bone defect of AOI was measured with Image-pro Plus 6.0 software and the data were analyzed using SPSS 19.0 software package. RESULTS： H-E staining showed the new bone formation on the both sides. The results of Gomori modified trichrome staining showed significantly increased new bone formation after application of β-NGF at 21 d and 28 d. The quality of mature bone was significantly higher in the experiment group compared with the control group at 28 d （P〈0.05）. CONCLUSIONS： Local application of β-NGF during bone healing can promote new bone formation and bone remodeling.