中文摘要：

背景：咬合刺激对于颌骨的功能及改建至关重要，但人们对其调节骨移植修复骨缺损过程中骨改建的作用尚缺少清楚的认识。目的：应用形态学方法分析咬合刺激对胶原骨粉修复骨缺损中骨改建的可能调控作用。方法：在成年 SD 大鼠左侧下颌骨及顶骨区分别建立标准骨缺损模型，随即植入胶原骨粉充填骨缺损区。术后12周，通过X射线、苏木精-伊红染色、Gomori染色、抗酒石酸酸性磷酸酶染色及骨形成蛋白2免疫组织化学染色观察两骨缺损区的差异。结果与结论：下颌骨及顶骨骨缺损区均有新骨形成，顶骨骨缺损区的板层骨形成量明显多于下颌骨骨缺损区，新骨形成矿化度明显高于下颌骨骨缺损区，说明顶骨骨缺损区的骨改建优于下颌骨骨缺损区；顶骨骨缺损区抗酒石酸酸性磷酸酶及骨形成蛋白2积分吸光度值均低于下颌骨骨缺损区，说明顶骨骨缺损区的破骨细胞及成骨细胞活性低于下颌骨骨缺损区。表明咬合刺激可能通过影响骨的矿化及成熟而导致下颌骨骨缺损修复过程中骨改建的延迟。

英文摘要：

BACKGROUND：Occlusal stimulation is essential for mandible function and remodeling, but there is stil a lack of clear understanding about the effect of occlusal stimulation on the bone remodeling in the process of bone defect repair using bone grafts. OBJECTIVE：To analyze the possible regulative effect of occlusal stimulation on bone remodeling in the process of bone defect repair using colagen substitutes. METHODS：Standard models of bone defects were respectively established in left mandible and parietal bone area of adult Sprague-Dawley rats. Then the bone defects area were filed with colagen and bone meal. The differences of two bone defects areas were observed by X-ray, hematoxylin-eosin staining, Gomori staining, tartrate-resistant acid phosphatase staining and bone morphogenetic protein 2 immunohistochemical staining at the 12th week after operation. RESULTS AND CONCLUSION： New bone formation was visible in the bone defect regions of the mandible and parietal bone. The amount of lamelar bone formation and the degree of mineralization of the new bone were significantly increased in the parietal bone defect compared with the mandibular bone defect area, indicating the bone remodeling in the parietal bone defect area was better than that in the mandible bone defect area. The integral absorbance values of tartrate-resistant acid phosphatase and bone morphogenetic protein 2 in the parietal bone defect area were lower than those in the mandibular bone defect area, indicating that the viabilities＆nbsp;of osteoblasts and osteoclasts in the parietal bone defect area were lower than those in the mandible bone defect area. These results demonstrate that occlusal stimulation may delay the bone remodeling during the repair of mandibular bone defects by regulating bone mineralization and maturation.