通过温室盆栽试验,研究了添加发酵牛粪和造纸干粉对菌根化紫花苜蓿修复多环芳烃(PAHs)污染农田土壤的影响.结果发现,添加0.5%~2.0%发酵牛粪对紫花苜蓿根系丛枝菌根(AM)真菌侵染率无明显影响,但均显著提高了紫花苜蓿的植株生物量(p〈0.05),其中,添加1.0%和2.0%发酵牛粪处理的土壤PAHs含量较对照略有降低.添加0.05%和0.1%造纸干粉均显著提高了紫花苜蓿的AM真菌侵染率和植株生物量(p〈0.05),但添加0.2%造纸干粉却对紫花苜蓿的AM真菌侵染率和植株生物量产生了显著的抑制作用(p〈0.05),仅添加0.05%造纸干粉处理的土壤PAHs含量显著低于对照(p〈0.05),且3~5环PAHs降解率均得到显著提高(p〈0.05).此外,土壤中PAHs降解率与AM真菌侵染率之间呈线性回归关系(R^2=0.247,F=6.217,p〈0.05).以上结果表明,添加适量(0.5%~2%)发酵牛粪可通过直接增加养分供应来促进植物生长,但对PAHs降解影响较小;添加微量(0.05%~0.1%)造纸干粉可通过增进AM真菌侵染来促进植株生长,加速PAHs降解,因而可作为刺激性物质应用于菌根修复.
A greenhouse pot-experiment was carried out to investigate the effects of fermented cow dung (FD) and dry powdered sludge of papermaking waste (PP) on phytoremediation of PAH-contaminated farmland soils by arbuscular mycorrhizal fungi (AMF) infected alfalfa (Medicago sativa L.). The addition of 0.5% to 2.0% FD had no significant effect on AMF infection rate,but could promote growth of alfalfa (p〈0.05). PAH residues in the 1.0% and 2.0% FD treatments were lower than those in the control treatment. The addition of 0.05% and 0.1% PP enhanced AMF infection rate and plant biomass significantly (p〈0.05),while a negative effect was observed for the 0.2% treatment (p〈0.05). Significant degradation of PAH residues was observed only from the treatment with 0.05% PP (p〈0.05),and the degradation rates of PAHs with three,four or five-ring were higher than those of the control (p〈0.05). A linear regression relation was observed between PAH degradation rate in soil and the AMF infection rate in plant roots (R2=0.247,F=6.217,p〈0.05). These results indicated that the appropriate amount(0.5%~2%) of FD addition could increase nutrition to promote plant growth,but had little effect on the degradation of PAHs. However,small amounts(0.05%~0.1%) of PP accelerated AMF infection to improve plant growth and the degradation of PAHs,suggesting that it could be used as a stimulant in mycorrhizoremediation of PAH-contaminated soils.