通过温室盆栽试验,研究接种土著与外源丛枝菌根(AM)真菌对紫花苜蓿与黑麦草修复多环芳烃(PAHs)污染土壤的影响。结果表明,接种外源AM真菌——苏格兰球囊霉(Glomus caledonium)36号能够显著提高紫花苜蓿和黑麦草的AM真菌侵染率并促进植物生长,而接种土著菌剂或土著菌剂与36号菌剂双接种对AM真菌侵染和植物生长无促进作用,甚至降低了黑麦草苗期的AM真菌侵染率。种植紫花苜蓿和黑麦草促进了土壤中PAHs的降解,这2种植物接种36号菌剂的处理60天时土壤PAHs降解率分别达42.3%和41.1%,说明36号菌剂可以显著提高植物修复效率,而接种土著菌剂对修复作用无显著影响,土著菌剂与36号菌剂双接种对紫花苜蓿的修复效果也无显著影响,但60天时显著提高黑麦草的修复效率。土壤中PAHs降解率与植物根系的AM真菌侵染率呈显著正相关关系(P〈0.05),表明AM真菌侵染可以提高紫花苜蓿与黑麦草对PAHs污染土壤的修复效率。
A greenhouse pot-experiment was carried out to investigate effects of indigenous and exogenous arbuscular mycorrhizal fungi (AMF) on phytoremediation of PAHs-contaminated soil by Medicago sativa and Lolium muhiflorum. Inoculation of exogenous Glomus caledonium 36 enhanced significantly AMF infection rate of M. sativa and L. muhiflorum (P〈 0.05 ), and plant biomass as well, while inoculation of indigenous AMF alone or together with G. caledonium 36 did not show any positive effect on the AMF infection and plant biomass, and even decreased AMF infection rate of L. muhiflorum at its seedling stage. Planting M. sativa and L. muhiflorum stimulated PAHs degradation ( P 〈 0. 05 ) , which reached 42. 3% and 41.1% ,respectively on D60 after inoculation with G. caledonium 36, suggesting the significant effect of the strain. However, inoculation of indigenous AMF did not have such effect. Inoculation with indigenous AMF and G. caledonium together did not show much effect on M. sativa in the soil,but did on L. muhiflorum degrading PAHs on 960. A significant positive correlation was observed between degradation rate of PAHs in the soil and AMF infection rate in the plant roots (P〈0. 05 ), suggesting that AMF infection can increase the phytoremediation efficiency of PAHs-contaminated soil.