中文摘要：

笔者所在项目组的前期研究发现,蛹虫草深层培养可以产生至少两种纤溶酶.基于此,文中对蛹虫草深层培养产生的纤溶酶的分离纯化展开了研究.采用硫酸铵盐析、Sephadex G-25凝胶色谱、Phenyl-Sepharose HP疏水相互作用色谱、CM-Sepharose FF弱阳离子交换色谱和Superdex 75凝胶色谱对纤溶酶进行分离.最终纯化后的纤溶酶Ⅰ比活力达1467.44U/mg,纯化倍数为36.07,回收率为5.79%.纤溶酶Ⅱ比活力达1681.58U/mg,纯化倍数为41.33,回收率为4.00%.两种纤溶酶经电泳鉴定均达到电泳纯,相对分子质量分别约为28000和32000.

英文摘要：

In their earlier research,the authors have found that at least two kinds of fibrinolytic enzymes can be obtained from the submerged culture of Cordyceps militaris.As a further research,this paper deals with the isolation and purification of these fibrinolytic enzymes by means of ammonium sulfate precipitation,Sephadex G-25 gel filtration chromatography,Phenyl-Sepharose HP hydrophobic interaction chromatography,CM-Sepharose FF weak-cation exchange chromatography and Superdex 75 gel filtration chromatography.After the purification,two kinds of fibrinolytic enzymes are obtained,namely fibrinolytic enzyme I with a specific activity of 1 467.44 U/mg,a purification fold of 36.07 and a recovery of 5.79%,as well as fibrinolytic enzyme II with a specific activity of 1 681.58 U/mg,a purification fold of 41.33 and a recovery of 4.00%.SDS-PAGE results indicate that the two kinds of fibrinolytic enzymes,whose relative molecular mass are respectively about 28 000 and 32 000,are both electrophoretic pure.