中文摘要：

建立了高效液相色谱法测定3个唑来膦酸衍生物——1-羟基-3-（1￡卜咪唑-1-基）丙烷-1，1-双膦酸、1-羟基-4-（1H咪唑-1-基）丁烷-1，1-双膦酸和1-羟基·5-（1胁咪唑-1-基）戊烷-1，1-双膦酸。使用C．8色谱柱，以4mmol／L磷酸二氢钠缓冲液（pH5．3）-甲醇（30：70，含5mmol／L溴化四丁铵）为流动相，检测波长210nm。上述3个化合物在0．2～lmg／ml范围内线性关系良好。回收率分别为101．7％、100-3％和99．8％，RSD为1．26％、1．43％和1．61％。

英文摘要：

An HPLC method was established for the determination of three zoledronic acid derivatives： 1 -hydroxy-3- （1H-imidazol- 1-yl） propane- 1,1-diphosphonic acid, 1-hydroxy-4- （1H-imidazol- 1 -yl） butane- 1,1-di- phosphonic acid and 1-hydroxy-5-（1H-imidazol-l-yl）pentane-l,l-diphosphonic acid. A C18 column was used with the mobile phase of 4 mmol/L disodium hydrogen phosphate buffer （pH 5.3）-methanol [30 ： 70, containing 5 mmol/L tetrabutyl ammonium bromide （TBAB）] at the detection wavelength of 210 nm. The calibration curves were linear in the range of 0.2 - 1 mg/ml. Their recoveries were 101.7%, 100.3 % and 99.8%, with RSDs of 1.26%, 1.43 % and 1.61%, respectively.